Method for enzymatic repair of cementitious surfaces
An aqueous solution of carbonic anhydrase and calcium chloride is contacted with a cementitious surface defining at least one opening or fissure in the presence of ambient carbon dioxide to thereby cause the calcium chloride and carbon dioxide to react, whereby calcium carbonate precipitate from the solution and seals the opening or fissure.
1. A method of sealing a cementitious surface comprising the step of physically contacting at least one opening or fissure of a cementitious surface with an aqueous solution of carbonic anhydrase and calcium chloride, the aqueous solution is exposed to an ambient atmosphere that includes carbon dioxide, whereby calcium carbonate is formed by a chemical catalyzation process performed by the carbonic anhydrase using ambient carbon dioxide, and precipitates to thereby seal the opening or fissure.
2. The method of claim 1 , wherein the pH of the aqueous solution is in a range of between about 3.0 and about 9.5.
3. The method of claim 2 , wherein the pH is in a range of between about 6.5 and about 9.5.
4. The method of claim 1 , wherein the molar concentration of carbonic anhydrase of the aqueous solution is in a range of between 0.5 μM and about 1 mM.
5. The method of claim 4 , wherein the molar concentration of carbonic anhydrase of the aqueous solution is in a range of between about 1 μm and about 10 μM.
6. The method of claim 4 , wherein the molar concentration of calcium chloride is in a range of between about 0.5 M and about 5 M.
7. The method of claim 6 , wherein the molar concentration of calcium chloride is in a range of between about 1.5M and about 2.5M.
8. The method of claim 6 , wherein the temperature of the aqueous solution is between about 5° C. and about 95° C.
9. The method of claim 8 , wherein the temperature of the aqueous solution is between about 20° C. and about 40° C.
10. The method of claim 1 , wherein the aqueous solution has a carbonic anhydrase concentration of about I μM, a calcium chloride concentration of about 2M, a temperature of about 20° C., and a pH of about 8.0.