Use of miR-199a-5p, targets and/or inhibitors thereof for the diagnosis, prognosis and treatment of fibroproliferative disorders
The present invention relates to the use of the miRNA expression profile, particularly of miR-199a-5p, and the target genes regulated thereby for the diagnosis, prognosis and use of miR-199a-5p inhibitors for treating fibroproliferative disorders.
1. A method of treating a subject having a fibroproliferative disorder comprising
administering to the subject a miR-199a-5p inhibitor,
wherein the fibroproliferative disorder is idiopathic pulmonary fibrosis, and
wherein the miR-199a-5p inhibitor is a DNA analogue or an oligomer, and
wherein the DNA analogue or the oligomer consists of a contiguous sequence of 7 to approximately 23 nucleotides in length and inhibits the activity of miR-199a-5p.
2. The method according to claim 1 , wherein the miR-199a-5p inhibitor is an oligomer having a sequence complementary to miR-199a-5p.
3. The method according to claim 2 , wherein the oligomer consists of a seven nucleotide sequence complementary to the first 7 nucleotides located at the 5′ end of mature miR-199a-5p.
4. The method according to claim 1 , wherein the miR-199a-5p inhibitor is an oligomer comprising 8 to 23 contiguous nucleotides corresponding to reverse complement of a miR-199a-5p site present in CAV1 mRNA.
5. The method according to claim 1 , wherein the miR-199a-5p inhibitor is administered via an aerosol to respiratory epithelium of the subject.