Aptamer specifically binding to L-Ascorbic acid and use of the same
The present invention relates to a single-stranded DNA aptamer which inhibits oxidation by binding to L-ascorbic acid, characterized in that the single stranded DNA aptamer has at least one of stem-loop structure, has CG bond at the end of the stem-loop structure, and both of the beginning parts of the stem-loop structure are G or C, in which the same bases are faced to each other. The aptamer of the present invention has an anti-oxidative effect of vitamin C, it can be used for functional cosmetics of various formulations, oral nutritional supplements, foods, etc. by maintaining the reduced state of vitamin C using the aptamer which selectively binds to vitamin C to maintain the anti-oxidative function of vitamin C for a long time. In addition, it can be anticipated that the anti-oxidative effect is continued and maximized even with a small amount of vitamin C by using the aptamer selectively binding to vitamin C.
1. A single-stranded DNA aptamer which inhibits oxidation by binding to L-ascorbic acid,
the single stranded DNA aptamer having at least one
from 5′ to 3′ end direction CG-N1-XG step-loop structure,
wherein C is prior to an unpaired loop sequence G-N1-X in which N1 is any of 1 to 3 nucleotides and X is G or T, and G is subsequent to the unpaired loop sequence and forms a C-G bond with the C.
2. The single-stranded DNA aptamer of claim 1 , the DNA aptamer comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS: 1, 4, 5, 17, 22, 23 and 26.
3. An anti-oxidative composition comprising the aptamer of claim 1 as an active ingredient.
4. A method for inhibiting oxidation of vitamin C by treating the vitamin C with the aptamer of claim 1 .