Device, system and method for processing a sample

1. A method of processing a sample in a device having a single blister only, the blister defined between a first wall and a second wall, in which at least the first wall is flexible such that the blister can be divided into one or more sealed chambers by an external pressure applied to a portion of the first wall that urges the first wall towards the second wall, the method comprising:

applying a localised external pressure in the form of a two dimensional shape to urge the first wall towards the second wall to form first and second sealed chambers within the blister,

introducing a liquid sample to the first sealed chamber, and

removing the localised external pressure, so that the blister becomes undivided, and to open the seal between the first and second sealed chambers.

2. A method according to claim 1 , further comprising: preloading the blister with dried reagents held at predetermined locations and, after applying the localised external pressure, a first reagent is located in the first sealed chamber and a second reagent is located in the second sealed chamber.

3. A method according to claim 1 , further comprising: incubating, heating or mixing in the first sealed chamber before the seal is opened between the first and second sealed chambers.

4. A method according to claim 1 , wherein the localised external pressure is applied by a shaped platen or tool.

5. A method according to claim 1 , wherein more than two sealed chambers are formable within the blister.

6. A method according to claim 4 , wherein a plurality of tools or platens are used to apply the localized external pressure to form more than two sealed chambers within the blister.

7. A method according to claim 5 , further comprising: opening the seals separating each of the more than two sealed chambers in sequence to allow the sample to communicate with each chamber in order as it is processed.

8. A method according to claim 4 , further comprising: mixing the sample by oscillation of the tool or platen against the first wall.

9. A method according to claim 1 , wherein the device contains a liquid buffer or reagent contained in a sachet that is located in the blister, the liquid being releasable by the application of an external pressure to the sachet.

10. A method according to claim 1 , wherein the sample is moveable within the blister by the application of an external pressure from a tool or platen.

11. A method according to claim 1 , wherein the sample undergoes a protocol suitable for amplification of a nucleic acid or genetic material.

12. A method according to claim 1 further comprising: analysing a sample processed by the method.

13. A method according to claim 12 , wherein, the analysing comprises: contacting the processed sample with a test strip located within the device.

14. A method according to claim 1 , further comprising: loading the device into a machine for automatically performing processing on the sample.

15. A method according to claim 14 further comprising: removing the device containing a sample processed by the method from the machine; and

disposing of the sample.

16. A method according to claim 1 , further comprising: decontaminating the device.

17. A method according to claim 1 , wherein an external pressure is applied to a portion of the first wall to isolate or separate a predetermined volume of liquid within the blister.

18. A method according to claim 1 , further comprising:

applying a vacuum to the blister to facilitate introduction of the sample and/or transfer of liquid within the device and/or mixing of reagents and the sample and/or sealing portions of the device and/or locating objects within the device.

19. A method according to claim 1 in which the blister is sealed from the external environment.