Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics in response to cold
Methods and materials for modulating cold tolerance levels in plants are disclosed. For example, nucleic acids encoding cold tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased cold tolerance levels and plant products produced from plants having increased cold tolerance levels.
1. A method of producing a plant and/or plant tissue, said method comprising growing a plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide has 90% or greater sequence identity to the amino acid sequence of SEQ ID NO:2 and wherein a plant produced from said plant cell has an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said nucleic acid.
2. A method of increasing the level of cold tolerance in a plant, said method comprising introducing into a plant cell an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide has 90% or greater sequence identity to the amino acid sequence of SEQ ID NO:2 and wherein a plant produced from said plant cell has an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said exogenous nucleic acid.
3. A plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide has 90% or greater sequence identity to the amino acid sequence of SEQ ID NO:2, and wherein a plant produced from said plant cell has an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said nucleic acid.
4. The plant cell of claim 3 , wherein the polypeptide has 95 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:2, and wherein the plant produced from said plant cell exhibits increased growth under cold-stress condition as compared to the control plant and wild-type growth under standard condition.
5. The plant cell according to claim 3 , wherein the polypeptide has 97 percent or greater sequence identity to the amino acid sequence of SEQ ID NO:2.
6. The plant cell according to claim 3 , wherein the nucleotide sequence has 90% or greater sequence identity to SEQ ID NO:1.
7. A transgenic plant comprising the plant cell of claim 3 , wherein the plant exhibits increased growth under cold-stress condition as compared to the control plant and the plant further exhibits wild-type growth under standard condition.
8. The transgenic plant of claim 7 , wherein said polypeptide comprises SEQ ID NO:2.
9. The transgenic plant of claim 7 , wherein said plant is a member of a species selected from the group consisting of Panicum virgatum, Sorghum bicolor, Miscanthus giganteus, Saccharum sp., Populus balsamifera, Zea mays, Glycine max, Brassica napus, Triticum aestivum, Gossypium hirsutum, Oryza sativa, Helianthus annuus, Medicago sativa, Beta vulgaris , and Pennisetum glaucum.
10. A seed product comprising embryonic tissue from the transgenic plant according to claim 7 , wherein the seed product comprises the exogenous nucleic acid.