Method of diagnosis and prognosis
A method of measuring Gadd45β expression comprising the step of measuring Gadd45β expression levels in a sample of cells, for example CD 138 expressing cells, obtained from a subject known to have or suspected of having a haematological malignancy. Expression levels may be used in diagnosis, for example of multiple myeloma, in providing a prognosis, for example in a patient having multiple myeloma or in guiding selection of an appropriate treatment agent, especially a treatment agent comprising Gadd45β and or MKK7 inhibitors. Also datasets comprising measured expression levels from multiple subjects.
1. A method of treating a hematological malignancy in a subject in need thereof, the method comprising administering a Gadd45β inhibitor to the subject, wherein the Gadd45β expression level in a sample of CD138 positive cells from the subject is higher than in HEK-293T cells cultured in complete RPMI-1640 medium, and wherein the Gadd45β inhibitor is mDTP3.
2. The method of claim 1 , wherein the haematological malignancy is multiple myeloma.
3. The method of claim 1 , wherein the haematological malignancy is diffuse large B-cell lymphoma.
4. The method of claim 1 , wherein the sample of CD138 positive cells is a blood sample, or biopsy of bone marrow, lymph node, kidney, spleen or bone.
5. The method of claim 2 , wherein said Gadd45β expression levels is a mRNA levels as measured by RT-PCR or other nucleic acid amplification techniques or by a quantitative or semi-quantitative hybridisation technique.
6. The method of claim 2 , wherein said Gadd45β expression level is a protein levels as measured by flow cytometry, Western blotting, RIA, ELISA or other immunoassay.