Method for producing a protein phospholipid complex from a crustacean catch
A method of producing a protein phospholipid complex from a crustacean catch including protein is claimed where hydrolysis occurs without substantially denaturing the protein from the crustacean catch. A method of producing a stable protein phospholipid emulsion from a crustacean catch is also claimed. A method of producing crustacean oil is claimed where the oil is separated from the hydrolyzed crustacean catch. Other methods of producing a protein phospholipid complex are claimed which relate to not removing the shell, removing the shell, and removing then adding back the shell to form the protein phospholipid complex.
1. A method of producing polar krill oil comprising the steps of
a) disintegrating a krill catch to provide a disintegrated krill catch;
b) contacting the disintegrated krill catch with about 0.1-0.5% endoprotease mixture and about 0.01-0.05% exoprotease mixture by total weight of the disintegrated krill catch to provide a hydrolyzed krill catch; and
separating the hydrolyzed krill catch to provide a polar krill oil.
2. The method according to claim 1 , wherein the method is performed in the absence of an additional extraction step.
3. The method according to claim 1 , wherein the hydrolyzed krill catch is separated in step (c) with a filter, sieve, decanter, centrifuge, or a combination thereof.
4. The method according to claim 3 , wherein the hydrolyzed krill catch is separated in step (c) with a horizontal centrifuge.
5. The method according to claim 1 , wherein the ratio of endoprotease mixture to exoprotease mixture is about 10:1.
6. The method according to claim 1 , wherein the contacting of step (b) includes contacting with a cell wall degrading enzyme.
7. The method according to claim 6 , wherein the cell wall degrading enzyme is from the genus Aspergillus.
8. The method according to claim 7 , wherein the cell wall degrading enzyme is mylase, pectinase, xylanase, cellulose enzymes, or a combination thereof.
9. The method according to claim 1 , wherein the disintegrated krill catch is contacted with the enzymes for over 100 minutes to provide a hydrolyzed krill catch.
10. The method according to claim 1 , wherein said disintegrating comprises one or more of pulping, milling, grinding or shredding.
11. The method acccording to claim 1 , wherein said contacting comprises an organic solvent.
12. The method according to claim 1 , wherein said contacting comprises incubating the disintegrated krill catch at a temperature range of 45-75° C.