Method for detecting biological substance

1. A method of detecting a biological substance, comprising:

introducing a first substance dispersed in a solvent into a flow channel formed between a first substrate and a second substrate such that the first substance is placed in a first well formed in the first substrate;

after the introducing of the first substance, introducing into the flow channel a sealant which is immiscible with the solvent; and

detecting the first substance such that the biological substance is detected,

wherein the first substance is at least one of the biological substance and a polymer bead comprising the biological substance, the first well is formed such that the first well has an open side which faces downwards and is communicated with the flow channel, and the solvent has a specific gravity larger than a specific gravity of the first substance.

2. The method of claim 1 , wherein the solvent includes a signal amplification reaction reagent, and the detecting comprises performing a signal amplification reaction in the first well, and detecting a signal inside the first well.

3. The method of claim 2 , wherein the signal amplification reaction is an isothermal reaction.

4. The method of claim 3 , wherein the isothermal reaction is an enzyme reaction.

5. The method of claim 4 , wherein the enzyme reaction is an assay using a first oligonucleotide which binds to the biological substance, a second oligonucleotide which partially overlaps with the first oligonucleotide and forms an overlapping site when hybridized to the biological substance, and a structure-specific flap endonuclease which cleaves the second oligonucleotide at the overlapping site.

6. The method of claim 2 , wherein the detecting of the signal comprises detecting at least one of fluorescence, light emission, pH change, absorbance change, potential change, and electric current change, which correspond to presence or absence of the biological substance inside the first well.

7. The method of claim 1 , wherein the biological substance comprises one of DNA, RNA, miRNA, mRNA, and protein, and the solvent further includes a template nucleic acid as a labeling substance having a specific labeling ability for the biological substance or as a labeling substance in a bondable state.

8. The method of claim 7 , wherein the labeling substance includes at least one of a DNA chain that is different from the template nucleic acid, an enzyme, a particle, an antibody, and a liposome.

9. The method of claim 1 , wherein the first substance is the polymer bead comprising the biological substance.

10. A method of detecting a biological substance, comprising:

introducing a first substance dispersed in a solvent into a flow channel formed between a first substrate and a second substrate such that the first substance is placed in a first well formed in the first substrate;

introducing a second substance comprising a magnetic bead and dispersed in the solvent into the flow channel such that the second substance is placed in a second well formed in the second substrate; and

detecting the first substance such that the biological substance is detected,

wherein the first substance is at least one of the biological substance and a polymer bead comprising the biological substance, the first well is formed such that the first well has an open side which faces downwards and is communicated with the flow channel, and the solvent has a specific gravity larger than a specific gravity of the first substance and smaller than a specific gravity of the second substance, and the second well is formed in the second substrate such that the second well has an open side which faces upwards and is communicated with the flow channel.

11. The method of claim 10 , wherein the first substance is the polymer bead comprising the biological substance.

12. The method of claim 10 wherein the introducing of the second substance is at the same time as the introducing of the first substance.

13. The method of claim 10 , wherein the solvent includes a signal amplification reaction reagent, and the detecting comprises performing a signal amplification reaction in the first well, and detecting a signal inside the first well.

14. The method of claim 13 , wherein the signal amplification reaction is an isothermal reaction.

15. The method of claim 14 , wherein the isothermal reaction is an enzyme reaction.

16. The method of claim 15 , wherein the enzyme reaction is an assay using a first oligonucleotide which binds to the biological substance, a second oligonucleotide which partially overlaps with the first oligonucleotide and forms an overlapping site when hybridized to the biological substance, and a structure-specific flap endonuclease which cleaves the second oligonucleotide at the overlapping site.

17. The method of claim 13 , wherein the detecting of the signal comprises detecting at least one of fluorescence, light emission, pH change, absorbance change, potential change, and electric current change, which correspond to presence or absence of the biological substance inside the first well.

18. The method of claim 10 , wherein the biological substance comprises one of DNA, RNA, miRNA, mRNA, and protein, and the solvent further includes a template nucleic acid as a labeling substance having a specific labeling ability for the biological substance or as a labeling substance in a bondable state.

19. The method of claim 18 , wherein the labeling substance includes at least one of a DNA chain that is different from the template nucleic acid, an enzyme, a particle, an antibody, and a liposome.

20. The method of claim 10 , further comprising:

after the introducing of the first substance, introducing into the flow channel a sealant which is immiscible with the solvent.