Anti-microbial peptides and methods of use thereof
View Patent ↗Anti-microbial peptides and methods of use are provided.
1. An isolated anti-microbial peptide comprising the amino acid sequence GX 1 KRLVQRLKDX 2 LRNLV (SEQ ID NO: 58); wherein X 1 and X 2 are hydrophobic amino acids; wherein said peptide comprises a single amino acid substitution with any amino acid at any position other than X 1 or X 2 ; wherein said anti-microbial peptide retains anti-microbial activity with said substitution; and wherein said peptide optionally comprises at least one D-amino acid.
2. The isolated anti-microbial peptide of claim 1 , wherein X 1 and X 2 are biphenylalanine.
3. The isolated anti-microbial peptide of claim 1 , wherein said peptide comprises at least one D-amino acid.
4. The isolated anti-microbial peptide of claim 1 , wherein the amino acids at positions 5, 9, and 13 are D-amino acids.
5. The isolated anti-microbial peptide of claim 1 , wherein said peptide further comprises at least one modification selected from the group consisting of amidation and acetylation.
6. A composition comprising at least one anti-microbial peptide of claim 1 and at least one pharmaceutically acceptable carrier.
7. The composition of claim 6 , further comprising at least one antibiotic.
8. A method for inhibiting a microbial infection in a subject in need thereof, said method comprising administering to said subject the composition of claim 6 .
9. The method of claim 8 , further comprising the administration of at least one additional antibiotic.
10. The method of claim 8 , wherein said microbial infection is a staphylococcal infection.
11. The method of claim 8 , wherein said microbial infection is a methicillin-resistant Staphylococcus aureus (MRSA) infection.
12. An isolated anti-microbial peptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 68, SEQ ID NO: 69, and SEQ ID NO: 70.
13. An isolated anti-microbial peptide, wherein the peptide is selected from the group consisting of the peptide consisting of the amino acid sequence of SEQ ID NO: 68, the peptide consisting of the amino acid sequence of SEQ ID NO: 69, and the peptide consisting of the amino acid sequence of SEQ ID NO: 70.
14. The isolated anti-microbial peptide of claim 1 , wherein X 1 and X 2 are each independently selected from the group consisting of trifluoromethylphenylalanine, napthylalanine, and biphenylalanine.