Recombinant lubricin
Disclosed are new recombinant isoforms of human-like lubricin or PRG4 glycoprotein having outstanding lubrication properties and a novel glycosylation pattern, and methods for their manufacture at high levels enabling commercial production.
1. A recombinant lubricin glycoprotein expressed from a human PRG4 gene in a host cell culture, glycosylation of which is at least 90% core 1 glycosylation.
2. The recombinant lubricin glycoprotein of claim 1 , wherein the glycoprotein has a molecular weight of at least 450 kDa.
3. The recombinant lubricin glycoprotein of claim 1 , comprising at least 30% by weight glycosidic residues.
4. The recombinant lubricin glycoprotein of claim 1 , wherein the lubricin glycoprotein comprises at least 35% by weight glycosidic residues.
5. The recombinant lubricin glycoprotein of claim 1 , wherein at least 95% by weight of glycosylation of the lubricin glycoprotein is core 1 glycosylation.
6. The recombinant lubricin glycoprotein of claim 1 , wherein at least 99% by weight of glycosylation of the lubricin glycoprotein is core 1 glycosylation.
7. The recombinant lubricin glycoprotein of claim 1 , wherein the recombinant human lubricin glycoprotein is monomeric.
8. The recombinant lubricin glycoprotein of claim 1 , wherein the recombinant human lubricin glycoprotein is dimeric.
9. The recombinant lubricin glycoprotein of claim 1 , the glycosylation of which is at least 95% core 1 glycosylation.
10. The recombinant lubricin glycoprotein of claim 1 , the glycosylation of which is at least 99% core 1 glycosylation.