Ancestral virus sequences and uses thereof
View Patent ↗Methods are described for predicting ancestral sequences for viruses or portions thereof. Also described are predicted ancestral sequences for adeno-associated virus (AAV) capsid polypeptides. The disclosure also provides methods of gene transfer and methods of vaccinating subjects by administering a target antigen operably linked to the AAV capsid polypeptides.
1. An adeno-associated virus (AAV) capsid polypeptide having the amino acid sequence shown in SEQ ID NO: 42.
2. The AAV capsid polypeptide of claim 1 , wherein the AAV capsid polypeptide is purified.
3. The AAV capsid polypeptide of claim 1 encoded by the nucleic acid sequence shown in SEQ ID NO: 43.
4. A nucleic acid molecule encoding an adeno-associated virus (AAV) capsid polypeptide having the nucleic acid sequence shown in SEQ ID NO: 43.
5. A vector comprising the nucleic acid molecule of claim 4 .
6. A non-human host cell comprising the vector of claim 5 .
7. A purified virus particle comprising the AAV capsid polypeptide of claim 1 .
8. The purified virus particle of claim 7 , further comprising a transgene.
9. A method of gene transfer and/or vaccination with a transgene, the method comprising
administering a virus particle to a subject in need of gene transfer or vaccination, wherein the virus particle comprises an AAV capsid polypeptide having the amino acid sequence shown in SEQ ID NO:42.
10. A method of vaccinating a subject, the method comprising
administering a target antigen operably linked to a AAV capsid polypeptide having the amino acid sequence shown in SEQ ID NO:42 to a subject in need of vaccination.