Producing adipic acid and related compounds using hybrid polyketide synthases
The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic or hybrid module. The present invention also provides for a host cell comprising the PKS and when cultured produces the carboxylic acid. In some embodiments, the carboxylic acid is adipic acid.
1. A polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic module comprising SEQ ID NO:1 or SEQ ID NO:2, or a functional variant thereof comprising (i) an amino acid sequence at least 95% identical to SEQ ID NO:1 or SEQ ID NO:2, and (ii) the amino acid residues from position 1 to position 892 of SEQ ID NO:1, wherein the PKS is capable of synthesizing a carboxylic acid.
2. The PKS of claim 1 , wherein the functional variant thereof comprises an amino acid sequence at least 99% identical to SEQ ID NO:1 or SEQ ID NO:2.
3. The PKS of claim 2 , wherein the synthetic module comprises SEQ ID NO:1.
4. The PKS of claim 2 , wherein the synthetic module comprises SEQ ID NO:2.
5. The PKS of claim 1 , wherein the carboxylic acid is adipic acid.