α-amylase-binding nucleic acid molecule and use thereof
The present invention provides a novel nucleic acid molecule that can be used for detection of α-amylase. The α-amylase-binding nucleic acid molecule of the present invention is characterized in that it binds to α-amylase with a dissociation constant of 17 nM or less, and preferably includes a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 22, for example. According to the nucleic acid molecule of the present invention, it is possible to detect α-amylase in saliva.
1. An α-amylase-binding nucleic acid molecule comprising the following polynucleotide (a) or (c):
(a) a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 22; and
(c) a polynucleotide that consists of a base sequence having at least 80% sequence identity to any of the base sequences of the polynucleotide (a) and binds to the α-amylase.
2. The α-amylase-binding nucleic acid molecule according to claim 1 , wherein
the α-amylase-binding nucleic acid molecule comprises a modified base, which is a base modified with a modifying group.
3. The α-amylase-binding nucleic acid molecule according to claim 2 , wherein
the modified base is a modified thymine.
4. The α-amylase-binding nucleic acid molecule according to claim 2 , wherein
the modifying group is an adenine residue or a substituted adenine residue.
5. The α-amylase-binding nucleic acid molecule according to claim 4 , wherein
the substituted adenine residue has a substituent bound to N at a 9-position.
6. The α-amylase-binding nucleic acid molecule according to claim 5 , wherein
the substituent is an amidino aminoalkyl group.
7. The α-amylase-binding nucleic acid molecule according to claim 6 , wherein
the amidino aminoalkyl group is a 4-amidino aminobutyl group.
8. The α-amylase-binding nucleic acid molecule according to claim 2 , wherein
the modified base is a modified purine base with a 7-position of a purine base being modified with the modifying group or a modified pyrimidine base with a 5-position of a pyrimidine base being modified with the modifying group.
9. The α-amylase-binding nucleic acid molecule according to claim 2 , wherein
the polynucleotide is a DNA.
10. An α-amylase analysis sensor comprising:
the α-amylase-binding nucleic acid molecule according to claim 1 .
11. An α-amylase analysis method comprising the step of:
detecting α-amylase in a specimen by causing the specimen and a nucleic acid molecule to come into contact with each other,
wherein the nucleic acid molecule is the α-amylase-binding nucleic acid molecule according to claim 1 , and
in the detection step, the nucleic acid molecule is caused to bind to the α-amylase in the specimen, and the α-amylase in the specimen is detected by detecting the binding.
12. The α-amylase analysis method according to claim 11 , wherein
the specimen is at least one selected from the group consisting of saliva, urine, plasma, and serum.