Microfluidics based analyzer and method for fluid control thereof
The present disclosure relates to a microfluidic-based analyzer, including a drive module and a microfluidic disc. On the microfluidic disk, a capillary is connected between a mixing chamber and a waste chamber. More particularly, the capillary is connected to the mixing chamber through a first access on the first radius of the microfluidic disc, and the capillary is connected to the waste chamber through a second access on the second radius of the microfluidic disk. Specifically, a turn of the capillary is disposed between the first access and the second access, in which a folding is configured on a third radius of the microfluidic disc. Overall, the aforementioned microfluidic-based analyzer is able to be operated in different rotational speeds and is capable of evacuating the mixing chamber and enhancing the washing efficiency.
1. A microfluidic-based analyzer, comprising:
a drive module;
a microfluidic disc detachably configured on the drive module, wherein the microfluidic disc comprises:
at least one injection chamber;
at least one microfluidic structure connecting to the at least one injection chamber, comprising:
a mixing chamber connecting to the at least one injection chamber;
a capillary connecting to the mixing chamber; and
a waste chamber connecting to the capillary;
wherein the capillary comprises:
a first access connected to the mixing chamber, wherein the first access is configured on a first radius;
a second access connected to the waste chamber, wherein the second access is configured on a second radius; and
a turning section connected to the first access and the second access, wherein the turning section is configured on a third radius:
wherein the first radius is less than the second radius, and the third radius is less than the first radius.
2. The microfluidic-based analyzer as claimed in claim 1 , wherein the microfluidic structure comprises an overflow channel comprising:
a third access connected to the mixing chamber, wherein the third access is configured on a fourth radius; and
a fourth access connected to the waste chamber, wherein the fourth access is configured on the second radius;
wherein the fourth radius is less than the first radius.
3. The microfluidic-based analyzer as claimed in claim 2 , wherein the third radius is less than the fourth radius.
4. The microfluidic-based analyzer as claimed in claim 1 , wherein the mixing chamber comprises at least one magnetic bead.
5. The microfluidic-based analyzer as claimed in claim 1 , wherein each of the microfluidic structures further comprises at least one microfluidic valve, and each of the microfluidic valves respectively connects to each of the injection chambers and mixing chambers.
6. The microfluidic-based analyzer as claimed in claim 5 , wherein the microfluidic disc comprises a plurality of the microfluidic structures.
7. A microfluidic controlling method of a microfluidic-based analyzer, comprising:
providing the microfluidic-based analyzer as claimed in claim 1 ;
injecting a liquid into the microfluidic structure;
operating the drive module at a high rotational speed to control the liquid to flow into the mixing chamber, wherein a rotational speed of the drive module comprises a critical rotational speed, a first rotational speed, and a second rotational speed, the first rotational speed is less than the critical rotational speed, and the second rotational speed is greater than the critical rotational speed;
operating the drive module at a low rotational speed, wherein the drive module rotates at the first rotational speed and controls the liquid to flow into the second access by a capillary phenomenon; and
operating the drive module at the high rotational speed, wherein the drive module rotates at the second rotational speed, the drive module controls the liquid to penetrate the second access and to enter the waste chamber until the liquid in the mixing chamber is completely drained.
8. The microfluidic controlling method of the microfluidic-based analyzer as claimed in claim 7 , wherein the liquid comprises a stationary phase and a flowing phase.
9. The microfluidic controlling method of the microfluidic-based analyzer as claimed in claim 7 , wherein the critical rotational speed is:
ω
c
=
60
(
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sin
θ
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d
H
ρΔ
R
R
_
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0.5
.