Anti-bed bug monoclonal antibodies and methods of making and uses thereof
The present disclosure provides anti-bed bug monoclonal antibodies and antigen-binding fragments thereof as well as compositions and kits comprising the same. The present disclosure also provides methods of making monoclonal antibodies and antigen-binding fragments thereof and methods of using the same to detect bed bugs.
1. An antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
2. A monoclonal antibody or an antigen-binding fragment thereof comprising the three heavy and three light chain complementarity determining regions of the antibody of claim 1 .
3. The antibody or antigen-binding fragment of claim 2 , comprising the heavy and light chain variable regions of the antibody of claim 1 .
4. The antibody or antigen-binding fragment of claim 3 , comprising the heavy and light chains of the antibody of claim 1 .
5. The antibody or antigen-binding fragment of claim 1 , wherein the antibody or antigen-binding fragment is capable of binding to a bed bug antigen in a lysate of whole bed bugs or an extract of collection paper comprising bed bug waste material.
6. A conjugated monoclonal antibody or conjugated antigen-binding fragment, comprising the antibody or antigen-binding fragment of claim 1 and a detection agent.
7. The conjugated antibody or conjugated antigen-binding fragment of claim 6 , wherein the detection agent is colloidal gold.
8. The conjugated antibody or conjugated antigen-binding fragment of claim 6 , comprising the antibody produced by the hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2], or an antigen binding fragment thereof.
9. The conjugated antibody or conjugated antigen-binding fragment of claim 6 , comprising the antibody produced by the hybridoma deposited at the ATCC under Accession Number PTA-122645 [BB7], or an antigen binding fragment thereof.
10. A composition comprising the antibody or antigen-binding fragment of claim 1 .
11. A composition comprising:
(a) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], and
(b) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], or the conjugated antibody of claim 9 .
12. A composition comprising:
(a) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], and
(b) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], or the conjugated antibody of claim 8 .
13. A kit comprising the antibody or antigen-binding fragment of claim 1 .
14. A hybridoma capable of producing an antibody, wherein the hybridoma is deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7].
15. An isolated cell producing the antibody or antigen-binding fragment of claim 1 .
16. A method of making the antibody or antigen-binding fragment of claim 1 , comprising (a) culturing a cell that produces the antibody or antigen-binding fragment, and (b) isolating the antibody or antigen-binding fragment from the cultured cell.
17. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with the antibody or antigen-binding fragment of claim 1 , and detecting binding of the bed bug antigen to the antibody or antigen-binding fragment.
18. The method of claim 17 , wherein the detecting comprises performing a lateral flow assay.
19. The method of claim 17 , wherein the method further comprises collecting the sample comprising the bed bug antigen with a collection device and extracting antigens from the sample.
20. The method of claim 19 , wherein the collection device is a swab.
21. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], and the conjugated antibody of claim 9 .
22. A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], and the conjugated antibody of claim 8 .