Targeted shell for use in drug delivery system utilizing carbosilane dendrimer
The present invention is related to a targeted type shell for drug delivery system. The object of the present invention is to provide the targeted type shell for DDS, which comprises a carbosilane dendrimer containing a silole produced by which is formed by utilizing the reaction between thiol group and alkyl halide, and a targeted protein containing a labeled proteins such as green fluorescent protein with a target recognition site. The shell may incorporate compounds having a variety of molecular weight and biopolymers, and selectively deliver them into targeted cells.
1. A targeted type shell for drug delivery system comprising an aggregatable molecule shown in formula (I):
wherein,
each Y independently represents a bromine atom, a sulfur atom, or a target sequence presented,
wherein:
at least one Y is the target sequence presented part and is bound through a sulfur atom,
the target sequence presented part is composed of a protein or peptide having a target recognition site, and
provided that at least one Y is not bromine;
wherein the targeted type shell for drug delivery system encloses any molecule selected from the group consisting of a protein having molecular weight of 200,000 or less, a nucleic acid and a hydrophobic molecule.
2. The targeted type shell for drug delivery system according to the claim 1 , wherein said peptide specifically binds to any targeted protein selected from the group consisting of a surface antigen, a receptor, a gate, a transporter and a channel to form a conjugate for promoting endocytosis of said conjugate into a cell.
3. The targeted type shell for drug delivery system according to the claim 1 , wherein said peptide comprises the amino acid sequence selected from the group consisting of:
DMPGTVLPGG
(Sequence No. 1 in the sequence listing);
VPTDTDYSGG
(Sequence No. 2 in the sequence listing);
and
DMPGTVLPGG GGGSEGEWQ QQQHQWAKQE
(Sequence No. 3 in the sequence listing).
4. The targeted type shell for drug delivery system according to the claim 1 , wherein said peptide comprises the amino acid sequence selected from the group consisting of:
MASMTGGQQMGR DMPGTVLPGG MSKGEELFTG VVPILVELDG
DVNGHKFSVS GEGEGDATYG KLTLKFISTT GKLPVPWPTL
VTTLTYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF
KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK
LEYNYNSHNV YITADKQRNG IKANFKTRHN IEDGSVQLAD
HYQQNTPIGD GPVLLPDNHY LSTQSALLKD PNEKRDHMVL
LEFVTAAGSGIT DEVDGT ELYK GG HHHHHH
(Sequence No. 4 in the sequence listing);
MASMTGGQQMGR VPTDTDYSGG MSKGEELFTG VVPILVELDG
DVNGHKFSVS GEGEGDATYG KLTLKFISTT GKLPVPWPTL
VTTLTYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF
NDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK
LEYNYNSHNV YITADKQRNG IKANFKTRHN IEDGSVQLAD
HYQQNTPIGD GPVLLPDNHY LSTQSALLKD PNDKRDHMVL
LEFVTAAGSGIT DEVDGT ELYK GG HHHHHH
(Sequence No. 5 in the sequence listing);
and
MASMTGGQQMGR DMPGTVLPGG GGGSEGEWQQQQHQWAKQE
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG
KLTLKFISTT GKLPVPWPTL VTTLTYGVQC FSRYPDHMKR
HDFFKSAMPE GYVQERTISF KDDGNYKTRA EVKFEGDTLV
NRIELKGIDF KEDGNILGHK LEYNYNSHNV YITADKQRNG
IKANFKTRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY
LSTQSALLKD PNEKRDHMVL LEFVTAAGSGIT DEVDGTC ELYK GG
HHHHHH
(Sequence No. 6 in the sequence listing).
5. The targeted type shell for drug delivery system according to claim 1 , wherein the target sequence presented part is composed of a protein or peptide having a target recognition site thereby being delivered to a. targeted tissue, wherein said. targeted tissue is any tissue selected from the group consisting of a normal tissue having inflammation, a tissue having undesirable gene expressions, a cell having undesirable gene expressions, and a tissue composed of tumor cells.
6. The targeted type shell for drug delivery system according to claim 1 , wherein said aggregatable molecule shown in the formula (I) is selected from the group consisting of the following formula (II) to (V), wherein TSPP in the formula means fluorescent protein with said target recognition site:
7. The targeted type shell for drug delivery system according to claim 6 , wherein said protein is any fluorescent protein selected from the group consisting of a red fluorescent protein, a yellow fluorescent protein, a blue fluorescent protein and a green fluorescent protein.