Methods of detecting per cell PD-L1 expression and uses thereof
Methods are provided for detecting the per cell programmed-death ligand 1 (PD-L1) expression of neoplasia cells. Aspects of the methods include cytometrically assaying a labeled cell suspension to quantify per cell PD-L1 expression to detect whether a neoplastic cell that expresses PD-L1 above a predetermined threshold is present in the neoplasia sample. In addition, kits that find use in practicing the subject methods are also provided.
1. A method of cytometrically assaying whether a neoplastic cell that expresses a number of programmed-death ligand 1 (PD-L1) molecules above a predetermined threshold is present in a neoplasia sample, the method comprising:
contacting the neoplasia sample with a labeled binding member specific for PD-L1 to generate a labeled cell suspension; and
cytometrically assaying the labeled cell suspension to quantify the number of PD-L1 molecules per cell to detect whether a neoplastic cell that expresses the number of PD-L1 molecules above the predetermined threshold is present in the neoplasia sample.
2. The method according to claim 1 , wherein the cytometrically assaying further comprises assaying cell cycle.
3. The method according to claim 1 , wherein the cytometrically assaying further comprises assaying aneuploidy.
4. The method according to claim 1 , wherein the detected cell is proliferative.
5. The method according to claim 1 , wherein the labeling further comprises contacting the neoplasia sample with at least one labeled binding member specific for immune cells.
6. The method according to claim 1 , wherein the detected cell is a circulating tumor cell, a hematopoietic cancer cell, or a cell of a solid tumor.
7. The method according to claim 1 , wherein the predetermined threshold is 100 or more PD-L1 molecules per cell.
8. A method of treating a subject for a neoplasia, the method comprising:
cytometrically assaying whether a neoplasia in a subject is anti-programmed-death ligand 1 (PD-L1) and/or anti-programmed cell death protein 1 (PD-1) immunotherapy responsive, the method comprising:
contacting a cell suspension sample prepared from the neoplasia with a labeled binding member specific for PD-L1 to generate a labeled cell suspension;
cytometrically assaying the labeled cell suspension to quantify the number of PD-L1 molecules per cell to detect whether a population of cells that each expresses a level of PD-L1 molecules per cell that exceeds a predetermined threshold is present to identify whether the neoplasia is anti-PD-1/PD-L1 immunotherapy responsive; and
administering an anti-PD-1/PD-L1 immunotherapy to a subject comprising an anti-PD-1/PD-L1 immunotherapy responsive neoplasia.
9. The method according to claim 8 , wherein the population of cells is aneuploid.
10. The method according to claim 9 , wherein the aneuploid cells indicate the presence of circulating tumor cells in the subject.
11. The method according to claim 8 , wherein the method further comprises cytometrically assaying the labeled cell suspension to detect whether proliferative immune cells are present.
12. A method of treating a subject for a neoplasia, the method comprising:
administering an anti-PD-1/PD-L1 immunotherapy to a subject comprising an anti-PD-1/PD-L1 immunotherapy responsive neoplasia, wherein the neoplasia is identified as anti-PD-1/PD-L1 immunotherapy responsive according to the method of assaying whether a neoplasia in a subject is anti-programmed-death ligand 1 (PD-L1) and/or anti-programmed cell death protein 1 (PD-1) immunotherapy responsive recited in claim 8 .
13. The method according to claim 1 , wherein the neoplasia sample is prepared from a biopsy.
14. The method according to claim 1 , wherein the labeled binding member is a fluorescently labeled binding member specific for PD-L1 and is selected from the group consisting of an anti-PD-L1 antibody, and an anti-PD-L1 aptamer.
15. The method according to claim 5 , wherein the at least one labeled binding member specific for immune cells comprises a fluorescently labeled binding member specific for lymphocyte marker CD45 or CD8.
16. The method according to claim 8 , wherein the cytometrically assaying further comprises assaying cell cycle or assaying aneuploidy.
17. The method according to claim 8 , wherein the predetermined threshold is 100 or more PD-L1 molecules per cell.
18. The method according to claim 8 , wherein the cell suspension sample is prepared from a biopsy.
19. The method according to claim 8 , wherein the labeled binding member and specific for PD-L1 is selected from the group consisting of an anti-PD-L1 antibody, and an anti-PD-L1 aptamer.
20. The method according to claim 8 , wherein the labeling further comprises contacting the cell suspension sample with at least one labeled binding member specific for immune cells and wherein the at least one labeled binding member specific for immune cells comprises a labeled binding member specific for lymphocyte marker CD45 or a labeled binding member specific for lymphocyte marker CD8.