Polymer/copolymer nanoparticles conjugated to gambogic acid
A composition comprising a nanoparticle, wherein the nanoparticle comprises a polymer/copolymer conjugated to a moiety is disclosed. A method of forming a nanostructure includes stirring poly(lactide-co-glycolide (PLGA) and 11-Ethyl-3-(dimethylaminopropyl) carbodiimide (EDC) in CH 2 Cl 2 to create a PLGA mixture, n-boc-ethyelenediamine and N,N-Diisopropylethylamine (DIEA) are added to the PLGA mixture to create a reaction mixture. The reaction mixture is then precipitated in cold diethyl ether to form a purified polymer, which is then dried. The dried and purified polymer is then reconstituted in CH 2 Cl 2 :TFA solution and stirred under inert conditions. The product of the reconstituting step is evaporated to form a clear viscous residue that is dissolved in CH 2 Cl 2 and then precipitated in cold ether to form a polymer. These functional polymers can encapsulate a variety of bioactives forming nanosystems improving the performance of bioactives.
1. A composition comprising a nanoparticle, wherein the nanoparticle comprises a polymer/copolymer conjugated to gambogic acid.
2. The composition of claim 1 , wherein a concentration of the polymer/copolymer may be adjusted to suit a specific target.
3. The composition of claim 1 , wherein the composition possesses target specificity.
4. The composition of claim 1 , wherein the polymer/copolymer are polylactide, poly(lactide-co-glycolide) or polycaprolactone.
5. The composition of claim 1 , wherein the composition facilitates absorption of a desired bioactive.
6. The composition of claim 1 , wherein surface expression of the nanoparticle makes the polymer/copolymer conjugated to gambogic acid accessible to respective receptors on a target site.
7. The composition of claim 1 , wherein the composition expresses covalently bound ligands to interact non-competitively with receptors on cell membranes for receptor-mediated uptake.
8. The composition of claim 1 , wherein the composition comprises a ligand with amine, carboxyl or hydroxyl functionality.
9. The composition of claim 1 , wherein the a conjugated polymer/copolymer can form stable nanoparticles via emulsion-diffusion techniques.
10. The composition of claim 1 , wherein the gambogic acid is linked to carboxyl end groups of the polymer/copolymer via a linker.
11. The composition of claim 10 , wherein the linker is an ethylenediamine linker.
12. The composition of claim 1 , wherein the composition includes more than one ligand to tailor a distribution profile of the composition in a subject based on needs of a disease and/or drugs encapsulated.
13. A method of producing the composition of claim 1 , the method comprising:
synthesizing a copolymer conjugated with gambogic acid; and
preparing the nanoparticles by utilizing an emulsion-diffusion-evaporation method to entrap a desired bioactive within the nanoparticles.