Microtome sectionable gel support structure and methods
A sectionable tissue sample support structure including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample. A method of orienting, processing, embedding and microtome slicing a tissue sample using a gel compound preformed into a self supporting geometric shape. A combination including the sectionable tissue sample support structure and a package containing the sectionable tissue sample support structure.
1. Apparatus comprising:
a three dimensional, self-supporting and preformed gel block having a predetermined geometric shape defined by an outer boundary for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample, the gel block being sectionable by a microtome to form ribbon-like sections of the gel block and tissue sample;
preformed tissue retaining structure comprising a tissue sample receiving space formed in a portion of the three dimensional, self-supporting and preformed gel block, the preformed tissue sample receiving space extending within the outer boundary of the gel block and configured to retain and orient the tissue sample, and
a package enclosing the gel block under clean, moisture-proof conditions.
2. The apparatus of claim 1 , wherein the gel block is resilient such that after deformation from an original shape, the gel compound reverts back to the original shape.
3. The apparatus of claim 1 , wherein the tissue receiving space comprises at least one of: a preformed slit, a preformed hole, a preformed recess, or combinations thereof.
4. The apparatus of claim 1 , wherein the tissue retaining structure further comprises at least one deformable portion of the gel block configured to apply a force to the tissue sample and thereby retain the tissue sample in a desired orientation.
5. The apparatus of claim 4 , wherein the deformable portion further comprises a hinged jaw element configured to move between open and closed positions and apply a clamping force to the tissue sample in the closed position to thereby maintain the tissue sample in a desired orientation.
6. The apparatus of claim 1 , wherein the gel block is permeable to fluids and reagents used in processing the tissue sample to thereby provide full cross-sectional preservation of the tissue sample.