Detergent compositions comprising polypeptide variants

1. A detergent composition comprising i) a DNase variant having DNase activity selected from the group consisting of:

a) a DNase variant which compared to a DNase with SEQ ID NO: 1, comprises one or more substitutions selected from the group consisting of: S27K, L33R, L33V, L33Y, S39C, S66W wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has at least 60% sequence identity to the amino acid sequence shown in SEQ ID NO: 1;

b) a DNase variant which compared to a DNase with SEQ ID NO: 27, comprises one or more substitutions selected from the group consisting of: G4K, S7G, K8R, S9I, N16G, S27K, S27R, D32F, D32I, D32L, D32R, D32V, L33H, L33R, L33K, L33V, L33Y, S39C, G41P, S42H, D45E, Q48D, N61E, T65M, T65W, S66R, S66M, S66W, S66Y, S66V, F78L, P91L, S101N, S106L, S106R, S106H, Q109E, A112E, T127P, 5130A, 5130Y, T138D, Q140V, Q140G, A147P, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, L177Y, S179L and C180A, wherein each position corresponds to the positions of SEQ ID NO: 27 (numbering according to SEQ ID NO: 27) and wherein the variant has at least 60% sequence identity to the amino acid sequence shown in SEQ ID NO: 27; and

c) a DNase variant which compared to a DNase with SEQ ID NO: 28, comprises one or more substitutions selected from the group consisting of: G4K, S7G, K8R, S9I, N16G, S27K, S27R, D32F, D32I, D32L, D32R, D32V, L33H, L33R, L33K, L33V, L33Y, S39C, G41P, S42H, D45E, Q48D, N61E, T65M, T65W, S66R, S66M, S66W, S66Y, S66V, F78L, P91L, S101N, S106L, S106R, S106H, Q109E, A112E, T127P, 5130A, 5130Y, T138D, Q140V, Q140G, A147P, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, L177Y, S179L and C180A, wherein each position corresponds to the positions of SEQ ID NO: 28 (numbering according to SEQ ID NO: 28), and wherein the variant has at least 60% sequence identity to the amino acid sequence shown in SEQ ID NO: 28 and

ii) from 0.01 to 99.9 wt % of an adjunct.

2. A detergent composition according to claim 1 wherein the DNase variant comprises one or both motifs [D/M/L][S/T]GYSR[D/N] (SEQ ID NO: 25) and/or ASXNRSKG (SEQ ID NO: 26).

3. A detergent composition according to claim 1 wherein the DNase variant has at least one improved property, compared to the parent and/or compared to the DNase having the polypeptide shown in SEQ ID NO: 1, SEQ ID NO: 27 or SEQ ID NO: 28.

4. A detergent composition according to claim 3 wherein the improved property is residual activity ratio (RAR) being above 1 in at least one assay A, B or C.

5. A detergent composition according to claim 1 wherein the combination of mutations in the DNase variant provides an additive or synergistic effect.

6. A detergent composition according to claim 1 wherein the DNase variant has at least 70% but less than 100%, sequence identity to the DNase comprising the amino acid sequence shown in SEQ ID NO: 1.

7. A detergent composition according to claim 1 wherein the DNase variant has from 3 to 20 substitutions compared to SEQ ID NO: 1.

8. A detergent composition according to claim 1 wherein the DNase variant is a variant of a parent DNase selected from the group of polypeptides consisting of:

a) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 1,

b) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 2,

c) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 3,

d) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 4,

e) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 5,

f) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 6,

g) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 7,

h) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 8,

i) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 9,

j) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 10,

k) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 11,

l) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 12,

m) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 13,

n) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 14,

o) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 15,

p) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 16,

q) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 17,

r) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 18,

s) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 19,

t) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 20,

u) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 21,

v) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 22,

w) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 23, and

x) a polypeptide having at least 60% sequence identity to the polypeptide shown in SEQ ID NO: 24.

9. A detergent composition according to claim 1 , wherein the DNase variant is a variant of a parent DNase obtained from bacillus genus.

10. A detergent composition according to claim 9 , wherein the DNase variant is a variant of a parent DNase obtained from Bacillus cibi, Bacillus horikoshii, Bacillus horneckiae, Bacillus idriensis, Bacillus algicola, Bacillus vietnamensis, Bacillus hwajinpoensis, Bacillus indicus, Bacillus marisflavi or Bacillus luciferensis.

11. A detergent composition according to claim 1 wherein the DNase variant comprises one or more substitutions corresponding to substitutions from the group consisting of: G4K, S7G, K8R, D32I, D32L, D32R, D32V, L33R, L33K, S39C, G41P, S42H, Q48D, T65M, T65W, S66M, S66W, S66Y, S66V, S101N, S106L, S106R, S106H, S130A, S130Y, T138D, Q140V, Q140G, A147H, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, T177Y, S179L and C180A (numbering according to SEQ ID NO: 1).

12. A detergent composition according to claim 1 wherein the adjunct comprises from 5 to 70 wt % surfactant comprising anionic and/or nonionic surfactant.

13. A detergent composition according to claim 1 wherein the adjunct comprises additional enzymes selected from: amylases, proteases, lipases, cutinases, mannanases, galactanases, cellulases, xyloglucanases and mixtures thereof.

14. A method of washing comprising:

(1) contacting an article or surface with an aqueous wash liquor comprising: (i) a DNase variant selected from:

a. a DNase variant which compared to a DNase with SEQ ID NO: 1, comprises one or more substitutions selected from the group consisting of: G4K, S7G, K8R, S9I, N16G, S27K, S27R, D32F, D32I, D32L, D32R, D32V, L33H, L33R, L33K, L33V, L33Y, S39C, G41P, S42H, D45E, Q48D, N61E, T65M, T65W, S66R, S66M, S66W, S66Y, S66V, F78L, P91L, S101N, S106L, S106R, S106H, Q109E, A112E, T127P, S130A, S130Y, T138D, Q140V, Q140G, A147H, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, T177Y, S179L and C180A wherein each position corresponds to the position of the polypeptide of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has at least 60% identity to the amino acid sequence shown in SEQ ID NO: 1;

b. a DNase variant which compared to a DNase with SEQ ID NO: 27, comprises one or more substitutions selected from the group consisting of: G4K, S7G, K8R, S9I, N16G, S27K, S27R, D32F, D32I, D32L, D32R, D32V, L33H, L33R, L33K, L33V, L33Y, S39C, G41P, S42H, D45E, Q48D, N61E, T65M, T65W, S66R, S66M, S66W, S66Y, S66V, F78L, P91L, S101N, S106L, S106R, S106H, Q109E, A112E, T127P, 5130A, 5130Y, T138D, Q140V, Q140G, A147P, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, L177Y, S179L and C180A, wherein each position corresponds to the positions of SEQ ID NO: 27 (numbering according to SEQ ID NO: 27) and wherein the variant has at least 60% sequence identity to the amino acid sequence shown in SEQ ID NO: 27; and

c. a DNase variant which compared to a DNase with SEQ ID NO: 28, comprises one or more substitutions selected from the group consisting of: G4K, S7G, K8R, S9I, N16G, S27K, S27R, D32F, D32I, D32L, D32R, D32V, L33H, L33R, L33K, L33V, L33Y, S39C, G41P, S42H, D45E, Q48D, N61E, T65M, T65W, S66R, S66M, S66W, S66Y, S66V, F78L, P91L, S101N, S106L, S106R, S106H, Q109E, A112E, T127P, 5130A, 5130Y, T138D, Q140V, Q140G, A147P, C148A, W154Y, T157V, Y159A, Y159R, G162C, Q174N, L177Y, S179L and C180A, wherein each position corresponds to the positions of SEQ ID NO: 28 (numbering according to SEQ ID NO: 28), and wherein the variant has at least 60% sequence identity to the amino acid sequence shown in SEQ ID NO: 28; and (ii) an adjunct; and

(2) optionally rinsing and

(3) drying the surface.