Bispecific antibody or antibody mixture with common light chains
The present application provides a bispecific antibody or an antibody mixture with common light chains and a preparation method therefor. the present application also provides a nucleic acid molecule encoding the antibody or the mixture, a recombinant vector and a recombinant cell comprising the nucleic acid molecule, as well as a detection and quantitation method for the antibody or the mixture.
1. An isolated anti-HER2 bispecific antibody or an antigen binding portion thereof, said bispecific antibody or antigen binding portion thereof comprising two heavy chains and two identical light chains, wherein:
each of said light chains comprises a variable region having an amino acid sequence selected from the group consisting of amino acid positions 1-107 of SEQ ID NO: 2-SEQ ID NO:4 and SEQ ID NO:6,
and one of said two heavy chains comprises a variable region having the amino acid sequence of SEQ ID NO:23; and the other one of said two heavy chains comprises a variable region having the amino acid sequence as set forth in SEQ ID NO: 24.
2. A composition comprising the bispecific antibody or the antigen binding portion thereof of claim 1 , and a pharmaceutically acceptable carrier or excipient.