IP Library › Granted Patent US 10,808,234
Granted Patent B2
US 10,808,234 · App. 16/402,172 · Granted Oct 20, 2020

Variant amylase enzyme compositions and methods

Inventors: Xiyun Zhang (San Ramon, CA); Jie Yang (Foster City, CA); Khin Oo (Daly City, CA); Goutami Banerjee (Hayward, CA); Tatsuya Fukushima (Fremont, CA); Eric Lin Hu (Millbrae, CA); Marielle De Jesus Palatino (Stockton, CA); Jijiao Zeng (Albany, CA); Imad N. Sawaya (Redwood City, CA)
Assignee: Fornia BioSolutions, Inc.
C12N9/2402A21D8/042C11D3/386C12P7/06C12P19/14C12Y302/01001
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Quick Facts
Patent No.
US 10,808,234
App. No.
16/402,172
Granted
Oct 20, 2020
Kind
B2
Abstract

The invention is directed to novel amylases.

Claims (11)

1. A composition comprising a variant amylase enzyme having amylase activity, wherein said variant amylase enzyme has at least one amino acid substitution that corresponds to an amino acid substitution in SEQ ID NO: 4 selected form the group consisting of D19K, Y67L, F83L, V107I, A113S, Y139F, Q271D, T272N, T272M, S275K, K280Q, Y306H, F317Y, G338D, V344L, L351Y, K383N, F384W and L396S, and wherein said variant amylase enzyme has at least 95% sequence identity to the polypeptide of SEQ ID NO:4.

2. The composition according to claim 1 , wherein said variant amylase enzyme has at least 98% sequence identity to the polypeptide of SEQ ID NO:4.

3. The composition according to claim 1 , wherein said variant amylase enzyme has one, two, three, four, five, six or seven of said amino acid substitutions.

4. The composition according to claim 1 , wherein said at least one amino acid substitution is selected from the group consisting of: Y67L, D19K, F83L, V107I/Q271D/F317Y/K383N, S275K/K383N, Y139F/K383N, V107I/Y306H/K383N, V107I/Y139F/Q271D/K383N, V107I/Y306H, V107I, Y306H/K383N, L351Y/K383N, Y306H, V107I/Y139F, S275K, Y139F, Y139F/Y306H, Y139F/S275K, F317Y, V107I/K383N, K383N, A113S/V344L/F384W, K280Q/V344L/L396S, T272N/V344L, A113S, G338D/F384W, A113S/V344L, F384W/L396S, L396S and T272M.

5. The composition according to claim 1 , wherein said at least one amino acid substitution is D19K or V107I/Y306H/K383N.

6. The composition according to claim 1 , wherein said amino acid substitution is D19K, and wherein said variant amylase enzyme has at least 95% sequence identity to the polypeptide of SEQ ID NO: 10.

7. The composition according to claim 1 , wherein said variant amylase enzyme has SEQ ID NO:10.

8. The composition according to claim 1 , wherein said amino acid substitutions are V107I/Y306H/K383N, and wherein said variant amylase enzyme has at least 95% sequence identity to the polypeptide of SEQ ID NO:9.

9. The composition according to claim 1 , wherein said variant amylase enzyme has SEQ ID NO:9.

10. A method of making a variant amylase enzyme, wherein said method comprises substituting one or more amino acids in the parent amylase enzyme of SEQ ID NO: 4 to obtain one or more amino acid substitutions selected from the group consisting of D19K, Y67L, F83L, V107I, A113S, Y139F, Q271D, T272N, T272M, S275K, K280Q, Y306H, F317Y, G338D, V344L, L351Y, K383N, F384W and L396S, wherein said variant amylase enzyme has amylase activity, and wherein said variant amylase enzyme has at least 95% sequence identity to the polypeptide of SEQ ID NO:4.

11. A method for hydrolysis of starch, wherein said method comprises contacting said starch with said variant amylase of claim 1 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 8, 2019
From: ZHANG, XIYUN; YANG, JIE; OO, KHIN; BANERJEE, GOUTAMI; FUKUSHIMA, TATSUYA; HU, ERIC LIN; PALATINO, MARIELLE DE JESUS; ZENG, JIJIAO; SAWAYA, IMAD N.
To: FORNIA BIOSOLUTIONS, INC.
Reel/Frame 049119/0174 →
Continuity (2)
Provisional Application 62666986 · May 4, 2018
Related Publication 20190338264A1 · Nov 7, 2019
Cited By (1)
US 12,559,791