Antigenic composition for detecting auto-antibody with specific response to exosomal protein EIF3A, and method for diagnosing liver cancer using antigenic composition
Provided are an autoantibody specifically binding to an exosomal protein EIF3A (Eukaryotic translation initiation factor 3 subunit A) or a fragment including an antigen-binding site (paratope) thereof, a hybridoma cell line producing the autoantibody, a polypeptide having an amino acid sequence of an antigenic determinant (epitope) specifically binding to the autoantibody, a composition for diagnosing liver cancer including an agent measuring an expression level of the autoantibody or the fragment including the antigen-binding site thereof, a kit for diagnosing liver cancer including the composition, and a method of providing information for diagnosis of liver cancer by using the composition. Further, provided is a method of screening for a therapeutic agent for liver cancer by using an expression level of the autoantibody. When anti-EIF3A autoantibody of the present invention is used as a diagnostic marker for liver cancer, the incidence of liver cancer may be diagnosed at a high level only by using non-invasive biological samples. Furthermore, liver cancer may be easily diagnosed by using only the amino acid sequence identified in the present invention, leading to the effective development of diagnostic products such as a diagnostic kit for liver cancer.
1. An autoantibody which specifically binds to EIF3A (Eukaryotic translation initiation factor 3 subunit A) protein or a fragment of EIF3A protein comprising a binding site to which the autoantibody binds,
wherein the autoantibody comprises a heavy chain variable region comprising a heavy chain CDR1, the amino acid sequence of which is set forth in SEQ ID NO: 24; a heavy chain CDR2, the amino acid sequence of which is set forth in SEQ ID NO: 25; and a heavy chain CDR3, the amino acid sequence of which is set forth in SEQ ID NO: 26; and a light chain variable region comprising a light chain CDR1, the amino acid sequence of which is set forth in SEQ ID NO: 27; a light chain CDR2, the amino acid sequence of which is set forth in SEQ ID NO: 28; and a light chain CDR3, the amino acid sequence of which is set forth in SEQ ID NO: 29.
2. The autoantibody of claim 1 , wherein the autoantibody binds to a peptide, the amino acid sequence of which the peptide is selected from the group consisting of the sequences set forth in SEQ ID NOs: 1 to 9.
3. The autoantibody of claim 1 , wherein the autoantibody is produced by a hybridoma cell line deposited under Accession NO. KCTC 12590BP.
4. A hybridoma cell line producing the autoantibody which specifically binds to EIF3A protein of claim 1 .
5. The hybridoma cell line of claim 4 , wherein the hybridoma cell line is deposited under Accession NO. KCTC 12590BP.
6. A polypeptide comprising:
(i) an amino acid sequence selected from the group consisting of the sequences set forth in SEQ ID NOS: 1 to 9, and
(ii) additional cysteines at both ends of the amino acid sequence,
wherein the polypeptide specifically binds to the autoantibody of claim 1 .
7. A composition for diagnosing liver cancer, comprising an agent useful for measuring an expression level of the autoantibody which specifically binds to EIF3A protein or the fragment of EIF3A of claim 1 , wherein the agent useful for measuring the expression level of the autoantibody is a polypeptide comprising an antigenic determinant peptide sequence, the amino acid sequence of which the polypeptide is selected from the group of sequences consisting of SEQ ID NOS: 1 to 9.
8. A kit for diagnosing liver cancer, comprising the composition of claim 7 .
9. The kit of claim 8 , wherein the kit is useful for performing a method selected from the group consisting of Western blotting, ELISA (Enzyme Linked Immunosorbent Assay), radioimmunoassay, radioimmunodiffusion, Ouchterlony immunodiffusion, rocket immunoelectrophoresis, immunohistostaining, immunoprecipitation assay, complement fixation assay, FACS, protein chip assay, and combinations thereof.
10. A composition for diagnosing liver cancer, comprising a polypeptide useful for measuring an expression level of the autoantibody of claim 1 , wherein the polypeptide has a sequence which comprises:
(i) an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 9, and
(ii) additional cysteines at both ends of the amino acid sequence, and
wherein the polypeptide specifically binds to said autoantibody.
11. A kit for diagnosing liver cancer, comprising the composition of claim 10 .