Fusion protein comprising antifreeze protein and human epidermal growth factor with increased anti-oxidant activity and skin cell proliferation effect and cosmetic composition for anti-wrinkle comprising the same as effective component
A fusion protein has the amino acid sequence of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8. A composition having the fusion protein may improve skin wrinkle with increased anti-oxidant activity and skin cell proliferation effect. It can be advantageously used as a raw material of a functional cosmetic having an excellent skin regeneration effect like improvement of skin wrinkles and skin whitening.
1. A fusion protein consisting of the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 6.
2. A gene encoding the fusion protein of claim 1 .
3. The gene according to claim 2 , wherein the gene consists of any one of the nucleotide sequences of SEQ ID NOS: 1 or 2.
4. A recombinant vector comprising the gene of claim 2 .
5. A host cell transformed with the recombinant vector of claim 4 .
6. A method for producing a fusion protein in a host cell, the method comprising transforming the host cell with the recombinant vector of claim 4 to overexpress the gene encoding the fusion protein.
7. The method according to claim 6 , wherein the host cell is E. coli.
8. A cosmetic composition comprising the fusion protein of claim 1 , and at least one selected from the group consisting of a lipid material, an organic solvent, a dissolution agent, a condensation agent, a gelling agent, a softening agent, an anti-oxidant, a suspension agent, a stabilizer, a foaming agent, an aroma, a surface active agent, water, an ionic or non-ionic emulsifier, a filler, a metal ion sequestering agent, a chelating agent, a preservative, vitamin, a blocking agent, a moisturizing agent, essential oil, a dye, a pigment, a hydrophilic activating agent, a lipophilic activating agent, a common auxiliary agent, and a carrier.
9. The fusion protein of claim 1 , wherein the amino acid sequence is SEQ ID NO: 5.
10. The fusion protein of claim 1 , wherein the amino acid sequence is SEQ ID NO: 6.
11. A method for proliferating skin cells, the method comprising applying a composition comprising the fusion protein of claim 1 to the skin.
12. A method for improving skin wrinkle, the method comprising applying a composition comprising the fusion protein of claim 1 to the skin.