Computational design of self-assembling cyclic protein homo-oligomers
Described herein are polypeptides capable of self-assembling to form homo-oligomers, and methods for designing such polypeptides.
1. An isolated polypeptide comprising the amino acid sequence that has at least 70% sequence identity to the amino acid sequence of SEQ ID NO: 40, wherein the polypeptide is capable of self-assembly into homo-oligomers.
2. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 75% sequence identity to the amino acid sequence of SEQ ID NO: 40.
3. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 40.
4. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 40.
5. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 40.
6. The polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence selected from the group consisting of SEQ ID NOS: 16-21, 34-35, 37, and 40.
7. The isolated polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 40.
8. The isolated polypeptide of claim 1 , wherein residues in bold font for SEQ ID NO: 40 are conserved in the polypeptide EEAELAYLLGELAYKLGEYRIAIRAYRIALKRDPNNAEAWYNLGNAYYKQGRYREAIEYYQKAL ELDPNNAEAWYNLGNAYYERGEYEEAIEYYRKALRLDPNNADAMQNLLNAKMREE (SEQ ID NO: 40).
9. An isolated polypeptide homo-oligomer comprising a plurality of polypeptides according to claim 8 .
10. The isolated polypeptide homo-oligomer of claim 9 , further comprising a plurality of polypeptides having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 39.
11. An isolated polypeptide homo-oligomer comprising a plurality of polypeptides according to claim 1 .
12. The isolated polypeptide homo-oligomer of claim 11 , further comprising a plurality of polypeptides having at least 97% sequence identity to the amino acid sequence of SEQ ID NO: 39.
13. The isolated polypeptide oligomer of claim 12 , wherein residues in bold font for SEQ ID NO: 39 are conserved
(SEQ ID NO: 39)
KYDGSKLRIGIL H A R W NAE II L AL VL GA L KRL Q EFGVK R ENII IET VPGSF
ELPYGSKLFVEKQKRLGKPLDAIIPIGVLIKGSTMHFEYICDSTTHQLMKL
NFELGIPVIFGVLTCLTDEQAEARAGLIEGKMHNHGEDWGAAAVEMATKF
N.