Compositions and methods for treating and preventing venom related poisoning
View Patent ↗This invention relates to compositions and methods for treating, ameliorating and preventing the toxic effects of venom poisoning. In particular, the invention provides compositions comprising carbon monoxide releasing molecules (CORM) or compositions comprising CORM and iron releasing molecules (IRM) for one or more of enhancing coagulation, reducing fibrinolysis, and/or inactivating venom related metalloproteinase activity in a subject suffering from or at risk of suffering from venom poisoning.
1. A method of protecting a subject from venom related fibrinogenolytic activity, comprising administering to the subject in need thereof a therapeutically effective amount of a composition comprising a carbon monoxide releasing molecule (CORM) wherein the CORM is selected from the group consisting of tricarbonyldichlororuthenium (II) dimer, tricarbonylchloro(glycinato)ruthenium (II), sodium boranocarbonate, dimanganese decacarbonyl, and iron pentacarbonyl, wherein the administering of the composition results in interaction of venom within the subject and the composition, wherein such interaction results in inactivation of fibrinogenolytic activity in the venom thereby protecting the subject from venom related fibrinogenolytic activity, wherein the composition is formulated for administration by an aerosol spray, an ointment, a bandage, a surgical dressing, a wound packing, a patch, autoinjector, a swab, a liquid, a paste, a cream, a lotion, a foam, a gel, an emulsion, a powder, or a needle.
2. The method of claim 1 , wherein the composition comprises between about 25 μM to about 200 μM of the CORM or 350-1000 mg of the CORM.
3. The method of claim 1 , wherein the composition further comprises an iron releasing molecule (IRM).
4. The method of claim 3 , wherein the administering results in release of carbon monoxide from the CORM and iron from the IRM.
5. The method of claim 3 ,
wherein the IRM is selected from the group consisting of ferric chloride, iron dextran, ferric gluconate, iron sucrose, ferumoxytol, ferric carboxymaltose, and iron isomaltoside.
6. The method of claim 1 , wherein the composition comprises between 5-25 mg of the IRM.
7. The method of claim 1 , wherein the composition is a pharmaceutical composition.
8. The method of claim 1 , wherein inactivation of fibrinogenolytic activity in the venom prevents one or more of
venom mediated catalysis of fibrinogen in the subject,
venom mediated degradation of plasma coagulation in the subject, and
venom mediated coagulopathy in the subject.
9. The method of claim 1 , wherein the venom is Crotalus related venom, wherein the Crotalus related venom is a venom from a Crotalus species selected from C. adamanteus, C. aquilus, C. atrox, C. basilicus, C. cerastes, C. durissus, C. enyo, C. horridus, C. intermedius, C. lannomi, C. lepidus, C. mitchellii, C. molossus, C. oreganus, C. polystictus, C. pricei, C. pusillus, C. ruber, C. scutulatus, C. simus, C. stejnegeri, C. tigris, C. tortugensis, C. totonacus, C. transversus, C. triseriatus, C. viridis , and C. willardi.
10. The method of claim 1 , wherein the venom is from one of the following: Naja naja (Indian cobra), Bothrops asper (Fur-de-lance), Agkistrodon piscivorus piscivorus, Agkistrodon contortrix contortrix, Agkistrodon contortrix laticinctus, Askistrodon contortix pictigaster, Agkistrodon piscivorus leucostoma, Agkistrodon contortrix mokasen, Northern Pacific rattlesnake, Arizona Black rattlesnake, Prairie rattlesnake, Red Diamond rattlesnake, Timber rattlesnake, Eastern Diamondback rattlesnake, and Southern Pacific rattlesnake.
11. The method of claim 1 , wherein the subject is a living mammal.
12. The method of claim 1 , wherein the subject is a human subject.