Method for differentiating pluripotent stem cells into desired cell type
Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
1. A method of differentiating a pluripotent stem cell into a neural cell, comprising a step of introducing a transcription factor comprising any one of the following (1) to (3) into a pluripotent stem cell of mammalian origin:
(1) five transcription factors selected from NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2;
(2) four transcription factors selected from NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2;
(3) three transcription factors selected from NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2,
wherein the neural cell comprises a motor nerve.
2. A method of differentiating a pluripotent stem cell into a neural cell according to claim 1 , wherein the motor nerve is a peripheral motor nerve.
3. A method of differentiating a pluripotent stem cell into a nerve cell according to claim 1 , wherein the transcription factor is an mRNA encoding amino acid sequences of the transcription factor, a synthetic mRNA encoding amino acid sequences of the transcription factor, a nucleic acid encoding amino acid sequences of the transcription factor, or a protein.