Particle measuring device and particle measuring method

1. A particle measuring device comprising:

a flow cell including a flow passage of sample fluid containing a particle;

a light source configured to output light;

an irradiation optical system configured to irradiate, with the light from the light source, the sample fluid in the flow passage;

a first imaging unit arranged at a position facing or opposite to a fluid flow direction in which the sample fluid flows in the flow passage and configured to capture, from an extension direction of the flow passage, first multiple still images of scattered light from the particle in a detection region in the flow passage, the light passing through the detection region;

a first particle movement amount specifying unit configured to specify a position of the particle in each of the first multiple still images and specify a first movement amount of the particle in a first direction by Brownian motion and a second movement amount of the particle in a second direction by Brownian motion based on the position of the particle in each of the first multiple still images, the second direction being vertical against the first direction, the first direction and the second direction being vertical against the fluid flow direction; and

a particle size specifying unit configured to specify particle size of the particle from the first movement amount in the first direction and the second movement amount in the second direction without correcting a particle movement amount depending on a flow of the sample fluid.

2. The particle measuring device according to claim 1 , further comprising:

a second imaging unit configured to capture, from the first direction, second multiple still images of the scattered light from the particle in the detection region of the flow passage through which the light passes; and

a second particle movement amount specifying unit configured to specify a position of the particle in each of the second multiple still images and specify the second movement amount of the particle in the second direction and a third movement amount of the particle in the fluid flow direction based on the position of the particle in each of the second multiple still images, wherein

the particle size specifying unit specifies the particle size of the particle from the first movement amount in the first direction, the second movement amount in the second direction and the third movement amount in the fluid flow direction.

3. The particle measuring device according to claim 2 , wherein

the first imaging unit and the second imaging unit have an identical frame rate, and are synchronized with each other to switch a frame, and wherein

the particle size specifying unit specifies, for an identical particle, one of the first multiple still images captured by the first imaging unit and one of the second multiple still images captured by the second imaging unit, thereby specifying the first movement amount in the first direction, the second movement amount in the second direction and the third movement amount in the fluid flow direction and specifying the particle size of the particle from the first movement amount in the first direction, the second movement amount in the second direction and the third movement amount in the fluid flow direction.

4. The particle measuring device according to claim 1 , further comprising:

a scattered light intensity specifying unit configured to specify a scattered light intensity equivalent value of the particle from the first multiple still images; and

an analysis unit configured to analyze a refractive index of the particle based on the particle size specified by the particle size specifying unit and the scattered light intensity equivalent value specified by the scattered light intensity specifying unit.

5. The particle measuring device according to claim 1 , wherein

the flow cell has a shape bent in an L-shape, and wherein

the first imaging unit is arranged at the position facing the fluid flow direction and captures the first multiple still images from the position facing the fluid flow direction in the detection region.

6. The particle measuring device according to claim 5 , wherein

the flow cell has a spherical dimple at a portion bent in the L-shape, and wherein

the first imaging unit captures, through the dimple, the first multiple still images of the scattered light from the particle in the detection region.

7. The particle measuring device according to claim 1 , further comprising:

an analysis unit configured to count, for each particle size specified by the particle size specifying unit, the number of particles having the each particle size to calculate a number concentration and calculate particle size distribution from the number concentration for each optional particle size range.

8. The particle measuring device according to claim 1 , wherein the first particle movement amount specifying unit is configured to associate the particle in each of the first multiple still images, specify a track of the particle across the first multiple still images thereby specifying the first movement amount in the first direction and the second movement amount in the second direction.

9. The particle measuring device according to claim 1 , wherein the first imaging unit is configured to capture the first multiple still images at a predetermined frame rate, and

the particle size specifying unit is configured to specify the particle size of the particle from the predetermined frame rate, the first movement amount in the first direction and the second movement amount in the second direction.

10. A particle measuring device comprising:

a flow cell including a flow passage of sample fluid containing a particle;

a light source configured to output light;

an irradiation optical system configured to irradiate, with the light from the light source, the sample fluid in the flow passage;

a first imaging unit arranged at a position facing a fluid flow direction in which the sample fluid flows in the flow passage; and

a particle size specifying unit, wherein

the first imaging unit captures, from the position facing the fluid flow direction in a detection region, multiple still images of scattered light from the particle in the detection region within a predetermined area from a bending position of the flow passage, the light passing through the detection region, and wherein

the particle size specifying unit specifies a position of the particle in each of the multiple still images, specifies a first movement amount of the particle in a first direction by Brownian motion and a second movement amount of the particle in a second direction by Brownian motion based on the position of the particle in each of the multiple still images, the second direction being vertical against the first direction, the first direction and the second direction being vertical against the fluid flow direction, and

the particle size specifying unit specifies a flow velocity component of the sample fluid in the first direction and the second direction in the detection region, corrects the first movement amount in the first direction and the second movement amount in the second direction by subtracting a movement amount by the flow velocity component from the first movement amount in the first direction and the second movement amount in the second direction, and specifies a particle size of the particle from the corrected first movement amount in the first direction and the corrected second movement amount in the second direction.

11. A particle measuring method comprising:

irradiating, with light from a light source, sample fluid in a flow passage included in a flow cell;

capturing, by a first imaging unit arranged at a position facing or opposite to a fluid flow direction in which the sample fluid flows in the flow passage, from an extension direction of the flow passage, multiple still images of scattered light from a particle in a detection region in the flow passage, the light passing through the detection region;

specifying a position of the particle in each of the multiple still images;

specifying a first movement amount of the particle in a first direction by Brownian motion and a second movement amount of the particle in a second direction by Brownian motion based on the position of the particle in each of the multiple still images, the second direction being vertical against the first direction, the first direction and the second direction being vertical against the fluid flow direction; and

specifying a particle size of the particle from the first movement amount in the first direction and the second movement amount in the second direction without correcting a particle movement amount depending on a flow of the sample fluid.