Guided injections for AAV gene transfer to muscle
The disclosure relates to methods for treating subjects with musculoskeletal diseases or with muscle wasting not associated with a musculoskeletal disease by gene transfer with recombinant adeno-associated viruses (rAAV) encoding myostatin inhibitors such as follistatin-344. The rAAV are administered prior to development of diffuse muscle fibrosis in a subject, or the rAAV administration avoids regions of muscle fibrosis in a subject.
1. A method of improving follistatin-344 gene expression in muscle and thereby increasing muscle fiber number and/or increasing muscle fiber diameter and enhancing muscle function in a subject in need thereof comprising the step of administering to the subject by one or more intramuscular injections a composition comprising infectious encapsidated recombinant adeno-associated viruses (rAAVs), each rAAV comprising a rAAV genome comprising AAV inverted terminal repeats flanking a polynucleotide encoding follistatin-344, wherein the polynucleotide is operatively linked to transcriptional control DNA and wherein the genome lacks AAV rep and cap DNA, and
wherein the administration is prior to development of diffuse muscle fibrosis in the subject or wherein the intramuscular injection or injections avoid regions of muscle fibrosis in the subject.
2. The method of claim 1 wherein the subject suffers from Becker muscular dystrophy.
3. The method of claim 2 , wherein the rAAV dose injected is at least about 6×10 11 viral genome per kilogram per leg (vg/kg/leg) or at least about 1.2×10 12 vg/kg/subject.
4. The method of claim 1 wherein the subject suffers from Duchenne muscular dystrophy.
5. The method of claim 4 , wherein the rAAV dose injected is at least about 2×10 11 vg/kg/leg or at least about 6×10 11 vg/kg/subject.
6. The method of claim 1 wherein the subject suffers from inclusion body myositis.
7. The method of claim 6 , wherein the rAAV dose injected is at least about 1.2×10 12 viral genome per kilogram per limb (vg/kg/limb).
8. The method of claim 1 , 2 , 3 , 4 , 5 , 6 or 7 wherein the rAAV administered is AAV1.CMV.FS344.
9. The method of claim 1 , 2 , 3 , 4 , 5 , 6 or 7 wherein the intramuscular injections are guided by magnetic resonance imaging or positron emission tomography.