Methods and compositions for plant pest control
The present invention provides methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. The present invention also provides for combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants.
1. A method for producing a tomato plant exhibiting an improvement in fungal disease resistance, the method comprising topically applying to a plant surface a composition that comprises:
a. at least one dsRNA molecule of 24 to about 95 nucleotides in length, wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51 or SEQ ID NO: 53, wherein the dsRNA molecule is not operably linked to a promoter or to a viral vector and wherein the dsRNA molecule does not become integrated into the plant chromosome; and
b. a transfer agent comprising an organosilicone preparation that conditions the tomato plant surface to permeation by the dsRNA molecule into plant cells, wherein said tomato plant exhibits an improvement in fungal disease resistance that results from suppression of an endogenous tomato Mildew Resistance Locus 0 (MLO) gene.
2. The method of claim 1 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51.
3. The method of claim 1 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 53.
4. The method of claim 1 , wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 124-130 or 131.
5. The method of claim 1 , wherein said composition comprises two or more of said dsRNA molecules.
6. The method of claim 1 , wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.
7. The method of claim 1 , wherein said composition further comprises a non-polynucleotide herbicidal molecule and said plant is resistant to said herbicidal molecule.
8. A tomato plant obtained by the method of claim 1 ; wherein the plant comprises the dsRNA molecule.
9. A processed product of said plant of claim 8 , wherein said processed product comprises the dsRNA and exhibits an improved attribute relative to a processed product of an untreated control plant and wherein said improved attribute results from said fungal disease resistance.
10. A composition comprising a dsRNA molecule of 24 to about 95 nucleotides in length, wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51 or 53, wherein said dsRNA is not operably linked to a promoter or to a viral vector; and,
a transfer agent comprising an organosilicone preparation that conditions a plant surface to permeation by the dsRNA molecule into plant cells.
11. The composition of claim 10 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51.
12. The composition of claim 10 , wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 124-130 or 131.
13. The composition of claim 10 , wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.
14. The composition of claim 10 , wherein said dsRNA molecule is not physically bound to a biolistic particle.
15. A method of making a composition comprising the step of combining at least:
a) a dsRNA molecule of 24 to about 95 nucleotides in length, wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51 or 53, wherein said dsRNA is not operably linked to a promoter or a viral vector; and,
b) a transfer agent, wherein the transfer agent is an organosilicone preparation that conditions a plant surface to permeation by the dsRNA molecule into plant cells.
16. The composition of claim 10 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 53.
17. The method of claim 15 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 51.
18. The method of claim 15 , wherein said dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 53.
19. The method of claim 15 , wherein the dsRNA molecule comprises a segment of at least 24 contiguous nucleotides that are identical or complementary to SEQ ID NO: 124-130 or 131.