Method for internally coating a liquid test sample collection device with at least one anticoagulant compound, and devices and kits related thereto

1. A method of using a liquid test sample dispensing device to dispense a patient's liquid test sample into a reaction vessel for conductance of at least one diagnostic assay, the method comprising the steps of:

(a) preparing a colloidal solution, the colloidal solution comprising at least one anticoagulant compound and at least one insoluble volatile compound, the colloidal solution being contained within a receptacle;

(b) placing a liquid test sample dispensing device into the receptacle, the liquid test sample dispensing device comprising:

a body, the body comprising at least one side, a first end, and a second end; and

a capillary portion, the capillary portion comprising a capillary having at least one outer surface, at least one inner surface, a first end, a second end, and an opening located at the second end of the capillary, wherein the first end of the capillary is disposed through the second end of the body, further wherein the capillary is in fluid contact with the colloidal solution such that a volume of the colloidal solution enters into and is retained within the capillary via the opening such that the colloidal solution is in fluid contact with the at least one inner surface of the capillary;

(c) removing the liquid test sample dispensing device from the receptacle and heating at least the capillary portion of the liquid test sample dispensing device to a temperature wherein the at least one insoluble volatile compound evaporates from the colloidal solution contained within the capillary, and further wherein granules of the at least one anticoagulant compound are deposited on the at least one inner surface of the capillary;

(d) drawing the patient's liquid test sample into the capillary of the liquid test sample dispensing device, wherein the capillary has the at least one anticoagulant compound's granules deposited on the at least one inner surface of thereof; and

(e) inserting the liquid test sample dispensing device containing the patient's collected liquid test sample into the reaction vessel for conductance of at least one diagnostic assay of the patient's collected liquid test sample.

2. The method of claim 1 , wherein the patient's liquid test sample is whole blood.

3. The method of claim 1 , wherein the at least one anticoagulant compound is selected from the group consisting of acenocoumarol, dicoumarol, ethyl bicoumacetate, atromentin, phenindione, phenprocoumon, tecafarin, and warfarin acenocoumarol, dicoumarol, ethyl bicoumacetate, atromentin, phenindione, phenprocoumon, tecafarin, and warfarin, coumarin, heparin and heparin derivatives, fondaparinux, idraparinux, rivaroxaban, apixaban, edoxaban, betrixaban, darexaban, letaxaban, and eribaxaban, hirudin, lepirudin, bivalirudin, argatroban, dabigatran, batraxobin, hementin, vitamin E, ethylenediaminetetraacetic acid (EDTA), citrate, oxalate, and combinations thereof.

4. The method of claim 1 , wherein the at least one insoluble volatile compound is selected from the group consisting of acetone, benzene, ethyl acetate, hexane, methyl acetate, toluene, and combinations thereof.

5. The method of claim 1 , wherein the capillary portion in step (c) is heated to a temperature ranging from about 55° C. to about 150° C.

6. The method of claim 5 , wherein the capillary portion is heated via a heater selected from the group consisting of a commercial-grade dryer, a vacuum dryer, and combinations thereof.

7. The method of claim 1 , further comprising the step of heating the granules of the at least one anticoagulant compound to a temperature that melts the granules and thereby forms a uniform coating of the at least one anticoagulant compound on the at least one inner surface of the capillary prior to the collection of the patient's liquid test sample.

8. The method of claim 7 , wherein the granules of the at least one anticoagulant compound is heated to a temperature ranging from about 170° C. to about 200° C.