Compositions and methods to promote wound healing
Compositions and methods to promote wound healing are described. The compositions and methods up-regulate X-box binding protein 1 (XBP1) and/or inositol-requiring enzyme-1 (IRE-1). In various embodiments, the compositions and methods can be used to promote wound healing in diabetic subjects.
1. A method of promoting healing of an impaired wound in a diabetic subject, the method comprising directly administering immediately after wounding in a single dose, an effective amount of a replication-impaired adenoviral vector comprising a nucleotide sequence encoding human X-box binding protein 1 (XBP1) consisting of the amino acid sequence as set forth in SEQ ID NO: 1 to the wound edge of the diabetic subject, and transiently expressing the XBP1, thereby accelerating wound closure and re-epithelialization of the wound in the diabetic subject.
2. The method of claim 1 , further comprising applying a wound dressing to the wound.
3. A method of promoting healing of an impaired wound in a diabetic subject, the method comprising directly administering immediately after wounding in a single dose, an effective amount of a replication-impaired adenoviral vector comprising a nucleotide sequence encoding the amino acid sequence encoding human inositol-requiring enzyme-1α (IRE-1α) as set forth in SEQ ID NO: 2 to the wound edge of the diabetic subject, and transiently expressing the IRE-1α, thereby accelerating wound closure and re-epithelialization of the wound in the diabetic subject.
4. The method of claim 3 , further comprising applying a wound dressing to the wound.
5. The method of claim 1 , wherein administering comprises injection of the adenoviral vector on to the wound edge.
6. The method of claim 3 , wherein administering comprises injection of the adenoviral vector on to the wound edge.