Surfactant adhesive composition
Provided herein is a surfactant adhesive protein comprising an amphiphilic peptide at the carbon or amine terminal of the protein, enabling homogeneous dispersion of several materials in various solvents and coating of the homogeneously dispersed particles on a hydrophilic or hydrophobic surface, particularly the homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of a mussel adhesive protein; the surface adhesive protein can be used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.
1. A surfactant adhesive protein comprising an amphiphilic peptide and an adhesive protein wherein the amphiphilic peptide is incorporated into the adhesive protein, wherein said adhesive protein is derived from mussel adhesive protein selected from the group consisting of FP-1 comprising at least one of the SEQ ID Nos: 1-3, FP-2 comprising SEQ ID No, 4, FP-3 comprising at least one of the SEQ ID NOs: 5-8, FP-4 comprising SEQ ID NO: 9, FP-5 comprising at least one of the SEQ ID NOs:10-13, FP-6 comprising SEQ ID NO: 14, FP-151 comprising at least one of the SEQ ID NOs; 15-17, FP-131 comprising SEQ ID NO:18 and FP-251 comprising SEQ ID NO:19, and wherein said amphiphilic peptide is selected from the group consisting of ARARADADARARADAD (SEQ ID NO: 20), EAEAKAKAEAEAKAKA (SEQ ID NO:21), QQRFQWQFEQQ (SEQ ID NO: 22), AEAEAKAK (SEQ ID NO:23), DPHHHWYHMHQH (SEQ ID NO: 24), HNWYHWWMPHNT (SEQ ID NO:25), HWKHPWGAWDTL (SEQ ID NO: 26), HWSAWWIRSNQS (SEQ ID NO: 27), DDWSHWWRAWNG (SEQ ID NO: 28), YTSPWWLAWYDP (SEQ ID NO:29), AWWEAFIPNSIT (SEQ ID NO:30) and KLWKKWAKKWLKLWKA (SEQ ID NO:31); wherein optionally, if present, tyrosine residues among said adhesive protein are chemically modified to form DOPA (3,4-dibydroxphenylalanine) or DOPA-quinone.
2. The surfactant adhesive protein according to claim 1 , wherein said amphiphilic peptide is incorporated into one or more sites selected from the group consisting of the C-terminus and N-terminus of said adhesive protein.
3. The surfactant adhesive protein according to claim 1 , wherein said surfactant adhesive protein is fused with a biofunctional peptide.
4. The surfactant adhesive protein according to claim 3 , wherein said biofunctional peptide is selected from the group consisting of an antimicrobial peptide, an antiviral peptide, an anticancer peptide, an anti-immune peptide, an anti-atopic peptide and an antithrombotic peptide.
5. The surfactant adhesive protein according to claim 4 , wherein said anticancer peptide is composed of an amino acid sequence selected from the group consisting of SEQ ID NOs. 32-34.
6. The surfactant adhesive protein according to claim 4 , wherein said antiviral peptide is composed of the amino acid sequence of SEQ ID NO. 35.
7. The surfactant adhesive protein of claim 1 , wherein tyrosine residues among the amino acid residues of the adhesive protein are chemically modified to form DOPA (3,4-dihydroxyphenylalanine).
8. The surfactant adhesive protein of claim 7 , wherein said DOPA is further chemically modified to convert to DOPA-quinone.
9. The surfactant adhesive protein of claim 7 , wherein said chemical modification is conducted by tyrosinase.
10. A silicone oil comprising the surfactant adhesive protein according to claim 1 and mica particles, finely dispersed in a hydrophilic solvent.
11. The silicone oil according to claim 10 , wherein said hydrophilic solvent is water or a water-containing solvent.