Supplementation of milk formulas with microvesicles isolated from milk
Provided are microvesicles isolated from milk, compositions thereof and uses for the preparation of milk formulas. Further provided are microvesicles, including exosomes and/or fat globules which encapsulate various miRNA molecules and uses thereof, as exemplified by supplementing milk formulas.
1. A composition comprising infant formula and microvesicles isolated from skim fraction of natural milk, wherein the microvesicles encapsulate or comprise miRNA molecules, wherein the miRNA molecules comprise miR-148a and miR-6073, wherein the microvesicles further encapsulate or comprise biologically active compounds, and wherein the composition substantially does not include cells present in natural milk.
2. The composition of claim 1 , wherein the natural milk is obtained from bovine, goat or human.
3. The composition of claim 1 , wherein the microvesicles comprise exosomes.
4. The composition of claim 1 , wherein the biologically active compounds are selected from nucleic acid molecules, lipids, proteins and peptides.
5. The composition of claim 1 , wherein the infant formula is devoid of animal derived fat other than the fat originating from the microvesicles.
6. The composition of claim 1 , wherein the infant formula does not comprise miRNA molecules other than miRNA molecules comprised within the microvesicles.
7. The composition of claim 1 , wherein the microvesicles are in hydrated or lyophilized form.
8. The composition of claim 1 , wherein the infant formula is formulated for oral administration to infants.
9. The composition of claim 1 , wherein the natural milk is pasteurized.
10. The composition of claim 1 , wherein the natural milk is not pasteurized.
11. A method for enriching an infant formula, the method comprising adding an effective amount of microvesicles isolated from skim fraction of natural milk to the infant formula, thereby obtaining an enriched infant formula composition, wherein the infant formula is devoid of animal derived fat, wherein the microvesicles encapsulate or comprise miRNA molecules, wherein the miRNA molecules comprise miR-148a and miR-6073, wherein the microvesicles further encapsulate or comprise biologically active compounds, and wherein the enriched infant formula composition substantially does not include cells present in natural milk.
12. A method of treating cancer in a subject in need thereof, the method comprising administering a composition comprising milk infant formula and microvesicles isolated from skim fraction of natural milk, wherein the microvesicles encapsulate or comprise miRNA molecules, wherein the miRNA molecules comprise miR-148a and miR-6073, wherein the microvesicles further encapsulate or comprise biologically active compounds, and wherein the composition substantially does not include cells present in natural milk.