Molecular detection/diagnosis reagent for tumor
The present invention discloses a tumor molecular detection/diagnostic reagent, which takes excrement as a detection sample and includes an SDC2 gene methylation detection reagent. The methylation level of the SDC2 gene detected in the excrement has an extremely high relevance to the onset of the colorectal cancer. The sensitivity of the SDC2 gene in the excrement is 87 percent and the specificity is up to 98 percent or even higher than that in tissue.
1. A tumor molecular detection/diagnostic reagent comprising an SDC2 gene methylation detection reagent,
wherein the SDC2 gene methylation detection reagent contains a first amplification primer and a second amplification primer, and
wherein said first amplification primer consists of the nucleotide sequence of SEQ ID NO: 3 and said second amplification primer consists of the nucleotide sequence of SEQ ID NO: 4.
2. The detection/diagnostic reagent according to claim 1 , wherein said tumor molecular detection/diagnostic reagent further comprises DNA capturing reagents.
3. The detection/diagnostic reagent according to claim 2 , wherein the DNA capturing reagents are capturing magnetic beads.
4. The detection/diagnostic reagent according to claim 3 , wherein the capturing magnetic beads comprise a capturing sequence for the SDC2 gene.
5. The detection/diagnostic reagent according to claim 4 , wherein the capturing sequence is selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2.
6. The detection/diagnostic reagent according to claim 3 , wherein the capturing magnetic beads comprise a capturing for a CpG island of the SDC2 gene.
7. The detection/diagnostic reagent according to claim 1 , wherein the SDC2 gene methylation detection reagent is a methylation detection reagent for a CpG island.
8. The detection/diagnostic reagent according to claim 1 , wherein said detection/diagnostic reagent further comprises a detection probe.
9. The detection/diagnostic reagent according to claim 8 , wherein the detection probe has a sequence selected from the group consisting of SEQ ID NO: 25 and SEQ ID NO: 26.
10. The detection/diagnostic reagent according to claim 1 , wherein the tumor is intestinal cancer or precancerous adenoma.
11. The detection/diagnostic reagent according to claim 10 , wherein the intestinal cancer is colorectal cancer.
12. A tumor detection/diagnostic kit, comprising the detection/diagnostic reagent according to claim 1 .
13. The tumor detection/diagnostic kit according to claim 12 , wherein the tumor is intestinal cancer or precancerous adenoma.
14. The tumor detection/diagnostic kit according to claim 13 , wherein the intestinal cancer is colorectal cancer.
15. A method of detecting an intestinal tumor in a human subject comprising:
obtaining a sample of excrement from a human subject;
extracting DNA from said sample of excrement;
amplifying the DNA using the tumor molecular detection/diagnostic reagent of claim 1 ; and
detecting the presence of an intestinal tumor in the human subject when the level of the amplification product is greater in comparison to the level of an amplification product in control excrement samples from human subjects that do not have intestinal tumors.