Liquid chromatography technique
Liquid chromatography techniques are disclosed. Specifically, the liquid chromatography technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid-contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150° C., pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, and combinations thereof.
1. A liquid chromatography technique, comprising:
providing a liquid chromatography system having a coated metallic fluid-contacting element; and
transporting a fluid to contact the coated metallic fluid-contacting element;
wherein the fluid has a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, and the fluid has a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, and wherein the fluid includes an analyte selected from the group consisting of tetracycline, N-hydroxypyridine-2-on, adenosine triphosphate, and deoxynucleotide monophosphate.
2. The liquid chromatography technique of claim 1 , wherein the coated metallic fluid-contacting element has a coating including carbon, silicon, oxygen, and hydrogen, and a stainless steel substrate.
3. The liquid chromatography technique of claim 1 , wherein the coated metallic fluid-contacting element has a coating including carbon, silicon, oxygen, hydrogen, and fluorine, and a stainless steel substrate.
4. The liquid chromatography technique of claim 1 , wherein the coated metallic fluid-contacting element has a coating including carbon, silicon, oxygen, hydrogen, and nitrogen, and a stainless steel substrate.
5. The technique of claim 1 , wherein the pressure for the urging of the fluid is greater than 120 MPa.
6. The technique of claim 1 , wherein the temperature of the fluid is greater than 400° C.
7. The technique of claim 1 , wherein the pH of the fluid is less than 2.
8. The technique of claim 1 , wherein the coated metallic fluid-contacting element is a coated metallic column has a diameter of less than 2.1 mm.
9. The technique of claim 1 , wherein the coated metallic fluid-contacting element has a length of less than 20 cm.
10. The technique of claim 1 , wherein the transporting of the fluid through the coated metallic fluid-contacting element is at a linear velocity of at least 8 mm per second.
11. The technique of claim 1 , wherein the fluid includes a solvent selected from the group consisting of water, acetonitrile, methanol, formic acid, phosphoric acid, tetrahydrofuran, trifluoroacetic acid, and combinations thereof.
12. The technique of claim 1 , wherein the liquid chromatography system comprises a stationary phase positioned within the coated metallic fluid-contacting element, the stationary phase including particles having a size of less than 3 micrometers, and the coated metallic column having a length of between 10 cm and 20 cm.
13. The technique of claim 1 , wherein the liquid chromatography system comprises a stationary phase positioned within the coated metallic fluid-contacting element, the stationary phase including particles having a size of less than 1.5 micrometers.
14. The technique of claim 1 , wherein the coated metallic fluid-contacting element is bioinert.
15. The technique of claim 1 , wherein the coated metallic fluid-contacting element is resistant to protein stiction.
16. The technique of claim 1 , wherein the coated metallic fluid-contacting element has a coating having a thickness of at least 400 nm.
17. The technique of claim 1 , wherein the coated metallic fluid-contacting element has a coating having a thickness of between 1,000 nm and 1,600 nm.
18. A liquid chromatography technique, comprising:
providing a liquid chromatography system having a coated metallic column, a stationary phase being positioned within the coated metallic column, the stationary phase including particles having a size of less than 1.5 micrometers
transporting a fluid through the coated metallic column, wherein conditions for the transporting of the fluid include the temperature of the fluid being greater than 150° C., pressure urging the fluid within the coated metallic column being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, or combinations thereof;
wherein the coated metallic column has a coating including carbon, silicon, oxygen, and hydrogen, and a stainless steel substrate, the pH of the fluid is less than 2, the coated metallic column has a diameter of less than 2.1 mm, the coated metallic column has a length of less than 20 cm, and the coated metallic component has a coating having a thickness of at least 400 nm;
wherein the transporting of the fluid through the coated metallic column is at a linear velocity of at least 8 mm per second;
wherein the fluid includes a solvent selected from the group consisting of water, acetonitrile, methanol, formic acid, phosphoric acid, tetrahydrofuran, trifluoroacetic acid, and combinations thereof; and
wherein the fluid includes an analyte selected from the group consisting of tetracycline, N-hydroxypyridine-2-on, adenosine triphosphate, and deoxynucleotide monophosphate.