Protein kinase polynucleotides and polypeptides for increasing abiotic stress tolerance, yield, biomass, growth rate, and/or vigor in a plant
Polynucleotides and isolated polypeptides, nucleic acid constructs comprising the isolated polynucleotides, transgenic plants expressing same and methods of using same for increasing abiotic stress tolerance, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, and/or nitrogen use efficiency of a plant are disclosed.
1. A method of increasing salinity stress tolerance of a plant, comprising over-expressing within the plant a polypeptide comprising an amino acid sequence which exhibits at least 98% sequence identity to the amino acid sequence set forth by SEQ ID NO: 678 as compared to a native plant of the same species which is grown under the same growth conditions, thereby increasing the salinity stress tolerance of the plant.
2. The method of claim 1 , wherein said polypeptide comprises conservative amino acid substitutions with respect to the polypeptide set forth by SEQ ID NO: 678.
3. The method of claim 1 , wherein said polypeptide is set forth by SEQ ID NO: 678 or 728.
4. A method of increasing salinity stress tolerance of a plant, comprising over-expressing within the plant a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 678, 728, 8535 and 8536 as compared to a native plant of the same species which is grown under the same growth conditions, thereby increasing the salinity stress tolerance of the plant.
5. The method of claim 1 , wherein said over-expression is performed by transforming the plant with an exogenous polynucleotide comprising a nucleic acid sequence encoding said polypeptide, wherein said exogenous polynucleotide is operably linked to a promoter for directing expression of said nucleic acid sequence in a plant cell.
6. The method of claim 5 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 205 and 267, or a codon-optimized sequence thereof.
7. The method of claim 1 , further comprising growing the plant over-expressing said polypeptide under the salinity stress.
8. The method of claim 1 , further comprising selecting the plant over-expressing said polypeptide for an increased salinity stress tolerance as compared to a native plant of the same species which is grown under the same growth conditions.
9. A method of producing seeds of a crop, comprising:
(a) selecting a parent plant over-expressing a polypeptide comprising an amino acid sequence which exhibits at least 98% sequence identity to the polypeptide set forth by SEQ ID NO: 678 as compared to a native plant of the same species which is grown under the same growth conditions, wherein said parent plant exhibits an increased salinity stress tolerance as compared to a native plant which is grown under the same growth conditions, and;
(b) growing a seed-producing plant from said parent plant resultant of step (a), wherein said seed-producing plant which over-expresses said polypeptide has said trait, and;
(c) producing seeds from said seed-producing plant resultant of step (b),
thereby producing seeds of the crop.
10. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence which exhibits at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 678 operably linked to a heterologous plant promoter for directing transcription of said nucleic acid sequence in a plant cell, wherein said amino acid sequence is capable of increasing salinity stress tolerance of a plant.
11. The nucleic acid construct of claim 10 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 205 and 267, or a codon-optimized sequence thereof.
12. The nucleic acid construct of claim 10 , wherein said promoter is a constitutive promoter.
13. The nucleic acid construct of claim 10 , wherein said promoter is a tissue specific promoter.
14. The nucleic acid construct of claim 10 , wherein said promoter is an inducible promoter.
15. A method of growing a crop, comprising growing a crop plant transformed with the nucleic acid construct of claim 10 , wherein said crop plant is derived from parent plants that have been transformed to express said polypeptide and that have been selected for an increased salinity stress tolerance as compared to a native plant which is grown under the same growth conditions, and said crop plant which comprises said nucleic acid construct has said increased salinity stress tolerance, thereby growing the crop.
16. A plant cell transformed with the nucleic acid construct of claim 10 .
17. The plant cell of claim 16 , wherein said plant cell forms part of a plant.
18. A transgenic plant comprising the nucleic acid construct of claim 10 .
19. The method of claim 1 , wherein said amino acid sequence is set forth by SEQ ID NO: 678.
20. The method of claim 9 , wherein said amino acid sequence is set forth by SEQ ID NO: 678.
21. The nucleic acid construct of claim 10 , wherein said amino acid sequence is set forth by SEQ ID NO: 678.
22. A plant cell transformed with the nucleic acid construct of claim 21 .
23. A method of producing a transgenic plant, comprising:
(a) transforming a cell of the plant with the nucleic acid construct of claim 10 ,
(b) generating a mature plant from said cell of the plant,
thereby producing the transgenic plant.
24. A method of producing a transgenic plant, comprising:
(a) transforming a cell of the plant with the nucleic acid construct of claim 21 ,
(b) generating a mature plant from said cell of the plant,
thereby producing the transgenic plant.
25. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 678, 728, 8535 and 8536 operably linked to a heterologous plant promoter for directing transcription of said nucleic acid sequence in a plant cell, wherein said amino acid sequence is capable of increasing salinity stress tolerance of a plant.
26. The nucleic acid construct of claim 25 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 205, 267, 5292 and 5293, or a codon-optimized sequence thereof.
27. The nucleic acid construct of claim 25 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 205, 267, 5292 and 5293.
28. A plant cell transformed with the nucleic acid construct of claim 25 .
29. A transgenic plant comprising the nucleic acid construct of claim 25 .
30. A method of producing a transgenic plant, comprising:
(a) transforming a cell of the plant with the nucleic acid construct of claim 25 ,
(b) generating a mature plant from said cell of the plant,
thereby producing the transgenic plant.