Compositions and methods for prolonged cell storage
View Patent ↗Provided in some aspects are methods and compositions for preserving platelets or other cells. In some embodiments, a platelet storage media or cell storage media may comprise a low molecular weight polyethylene glycol (e.g. PEG-400) to allow for extended storage and/or refrigeration of the platelets or cells.
1. A method for preserving platelets, comprising:
(a) admixing cells with a cell media comprising a polyethylene glycol having an average molecular weight of from 200 g/mol to 500 g/mol less in a concentration of from about 0.1% (wt/v) to about 4% (wt/v);
(b) wherein the method comprises storing the cell media comprising the platelets at a temperature of from about 0.1° C. to about 25° C.
2. The method of claim 1 , wherein the concentration of the polyethylene glycol is from about 1% to about 3%.
3. The method of claim 1 , wherein the concentration of the polyethylene glycol is from about 1.1% to about 2.5%.
4. The method of claim 1 , wherein the cells are platelets, and wherein the method allows for storage of the platelets for more than 5 days without irreversible aggregation of the platelets.
5. The method of claim 4 , wherein the method allows for storage of the platelets for at least 5-30 days without irreversible aggregation.
6. The method of claim 1 , wherein the temperature is from about 0.1-10° C.
7. The method of claim 6 , wherein the temperature is from about 0.1-5° C.
8. The method of claim 1 , wherein the polyethylene glycol is PEG-200, PEG-225, PEG-250, PEG-275, PEG-300, PEG-325, PEG-350, PEG-375, PEG-400, PEG-425, PEG-450, PEG-475, or PEG-500.
9. The method of claim 8 , wherein the cell media does not contain or only contains trace amounts of ethylene glycol, diethylene glycol, and/or triethylene glycol.
10. The method of claim 1 , wherein the cells are platelets, and wherein the cell media comprises or consists of Acid Citrate Dextrose (ACD) solution, the polyethylene glycol, and the platelets.
11. The method of claim 1 , wherein the media does not contain or only contains trace amounts of ethylene glycol, diethylene glycol, and/or triethylene glycol.
12. The method of claim 1 , wherein the media further comprises about 0.3-3% of a compound having the structure:
wherein:
R 1 , R 2 , and R 3 are each independently hydrogen, a polyethylene glycol comprising 1-5 repeating units, or a polypropylene glycol comprising 1-5 repeating units; and
R 4 is hydrogen, alkyl (C1-12) , substituted alkyl (C1-12) , or a —(CH 2 ) x —R 5 ; wherein:
x is 0, 1, 2, or 3; and
R 5 is a polyethylene glycol comprising 1-5 repeating units or a polypropylene glycol comprising 1-5 repeating units.
13. The method of claim 12 , wherein the compound is glycerol propoxylate, trimethylolpropane ethoxylate, pentaerythritol propoxylate, or pentaerythritol ethoxylate.
14. The method of claim 12 , wherein the compound is pentaerythritol propoxylate.
15. The method of claim 1 , wherein the cell media is comprised in an oxygen permeable polymer or plastic bag or container.
16. The method of claim 1 , wherein the method comprises storing the cell media comprising the platelets at a temperature of from about 0.1° C. to about 15° C.
17. The method of claim 1 , wherein the method comprises storing the cell media comprising the platelets at a temperature of from about 20° C. to about 25° C.
18. The method of claim 8 , wherein the polyethylene glycol is PEG-375, PEG-400, PEG-425, or PEG-450.
19. The method of claim 1 , wherein the polyethylene glycol has an average molecular weight of 200 g/mol to 420 g/mol.
20. The method of claim 1 , wherein the polyethylene glycol has an average molecular weight of 300 g/mol to 450 g/mol.