Methods and devices for producing cellular suspensions from tissue samples
Aspects of the present disclosure include methods of producing a cellular suspension from a tissue sample by applying resonant acoustic energy to a container comprising the tissue sample in a manner sufficient to produce a cellular suspension from the tissue sample. Resonant acoustic mixers and kits for use in producing a cellular suspension from a tissue sample are also provided.
1. A method of producing a cellular suspension from a tissue sample, the method comprising:
applying resonant acoustic energy to a container comprising the tissue sample at a frequency ranging from 20 Hz to 20,000 Hz to dissociate cells from the tissue sample and produce live dissociated cells in a cellular suspension from the tissue sample.
2. The method according to claim 1 , wherein the container is a sealed container.
3. The method according to claim 1 , wherein the tissue sample is a mammalian tissue sample.
4. The method according to claim 3 , wherein the tissue sample is a biopsy sample.
5. The method according to claim 4 , wherein the biopsy sample is a tumor biopsy sample.
6. The method according to claim 1 , wherein the method further comprises manipulating the cellular suspension.
7. The method according to claim 6 , wherein the manipulating comprises flow cytometrically processing the cellular suspension.
8. The method according to claim 7 , wherein the flow cytometrically processing the cellular suspension comprises sorting the cellular suspension.
9. The method according to claim 1 , wherein the method further comprises assaying a cell of the cellular suspension.
10. The method according to claim 9 , where the assaying comprises nucleic acid analysis.
11. The method according to claim 1 , wherein the container further comprises at least one of an enzymatic dissolution agent and a milling agent.
12. The method according to claim 1 , wherein the resonant acoustic energy is supplied by cyclic linear displacement of the container.