Method for screening agents promoting skin barrier function and method for evaluating skin barrier function taking epidermal serine racemase and/or D-serine level as indicator
The purpose of the present invention is to develop a method for screening drugs having the effect of increasing skin barrier function in in vitro studies and to evaluate barrier function in the skin. Candidate drugs can be screened by using the activity and/or expression level of serine racemase as an indicator.
1. A method for screening a skin barrier function enhancing agent, said method comprising:
a) providing a cell culture comprising keratinocytes,
b) adding a candidate agent to the cell culture comprising keratinocytes,
c) determining an expression level of serine racemase in said cell culture, and
d) comparing the expression level of serine racemase to a control expression level of serine racemase in a control cell culture, to which the candidate agent is not added,
wherein when the expression level of serine racemase in said cell culture is greater than the control expression level of serine racemase in said control cell culture, the candidate agent is a skin barrier function enhancing agent.
2. The method of claim 1 , wherein said determining the expression level of serine racemase comprises measuring the amount of serine racemase mRNA in the keratinocytes.
3. The method of claim 1 , wherein the keratinocytes undergo a differentiation inducing step by subjecting the keratinocytes to a differentiation-inducing stimulation.
4. The method of claim 3 , wherein the differentiation-inducing step is carried out before or after adding the candidate agent to the cell culture comprising keratinocytes or simultaneously with adding the candidate agent to the cell culture comprising keratinocytes.
5. The method of claim 3 , wherein the differentiation-inducing stimulation is selected from the group comprising calcium ion, phorbol-12-myristate-13-acetate, voltage, and gas phase exposure.
6. A method for screening a skin barrier function enhancing agent, said method comprising:
a) providing a cell culture comprising keratinocytes,
b) adding a candidate agent to the cell culture comprising keratinocytes,
c) determining a level of serine racemase activity in said cell culture,
d) comparing the level of serine racemase activity to a control level of serine racemase activity in a control cell culture, to which the candidate agent is not added, wherein when the level of serine racemase activity in said cell culture is greater than the control level of serine racemase activity in said control cell culture, the candidate agent is a skin barrier function enhancing agent.
7. The method of claim 6 , wherein said determining the level of serine racemase activity comprises measuring the substrate conversion efficiency of serine racemase in the keratinocytes.
8. The method of claim 6 , wherein said determining the level of serine racemase activity comprises measuring the amount of D-amino acid product in the keratinocytes.
9. The method of claim 6 , wherein the keratinocytes undergo a differentiation inducing step by subjecting the keratinocytes to a differentiation inducing stimulation.
10. The method of claim 9 , wherein the differentiation-inducing step is carried out before or after adding the candidate agent to the cell culture comprising keratinocytes or simultaneously with adding the candidate agent to the cell culture comprising keratinocytes.
11. The method of claim 9 , wherein the differentiation-inducing stimulation is selected from the group comprising calcium ion, phorbol-12-myristate-13-acetate, voltage, and gas phase exposure.
12. A method for screening a skin barrier function enhancing agent, said method comprising:
a) providing a cell culture comprising keratinocytes,
b) adding a candidate agent to the cell culture comprising keratinocytes,
c) determining a level of D-serine in said cell culture,
d) comparing the level of D-serine to a control level of D-serine in a control cell culture, to which the candidate agent is not added,
wherein when the level of D-serine in said cell culture is greater than the control level of D-serine in said control cell culture, the candidate agent is a skin barrier function enhancing agent.
13. The method of claim 12 , wherein the keratinocytes undergo a differentiation inducing step by subjecting the keratinocytes to a differentiation inducing stimulation.
14. The method of claim 13 , wherein the differentiation-inducing step is carried out before or after adding the candidate agent to the cell culture comprising keratinocytes or simultaneously with adding the candidate agent to the cell culture comprising keratinocytes.
15. The method of claim 13 , wherein the differentiation-inducing stimulation is selected from the group comprising calcium ion, phorbol-12-myristate-13-acetate, voltage, and gas phase exposure.