Device for real-time measurement of bacteria by atp detection
Provided is a device for real-time measurement of bacteria. The device for real-time measurement of bacteria includes reaction portions, a support portion configured to support the reaction portions, a rotational shaft configured to transfer the support portion, and a sample supply portion configured to supply a sample to each of the reaction portions, and according to the device for real-time measurement of bacteria, bacteria may be measured in real time through the detection of ATP.
1. A device for real-time measurement of bacteria, the device comprising:
at least two reaction portions, each having a reagent for adenosine triphosphate (ATP) detection immobilized thereon;
a flexible support portion configured to support the at least two reaction portions;
a rotational shaft connected to the support portion and configured to move the support portion; and
a sample supply portion configured to supply a sample to each of the reaction portions,
wherein the at least two reaction portions comprise a reagent for ATP detection comprising luciferase and luciferin and a member configured to dry and immobilize the reagent, and
wherein the at least two reaction portions are arranged on the support portion at regular intervals.
2. The device of claim 1 , wherein the reagent further comprises at least one of dithiothreitol (DTT), Tris, glycine, MgSO 4 , ethylenediaminetetraacetic acid (EDTA), or sodium azide.
3. The device of claim 1 , wherein the support portion is formed of a non-moisture permeable material.
4. The device of claim 1 , wherein the sample supply portion comprises a bacteria collector configured to collect bacteria and a bacteria lysis portion configured to lyse bacteria.
5. The device of claim 4 , wherein the bacteria collector collects airborne bacteria by exposing a solution to the air.
6. The device of claim 4 , wherein the bacteria lysis portion performs heat treatment on the collected bacteria.
7. The device of claim 1 , wherein the member configured to dry and immobilize the reagent is at least one of paper, natural fiber, synthetic fiber, or porous material.
8. The device of claim 1 , wherein the support portion and the at least two reaction portions are capable of being wound in a cylindrical form according to rotation of the rotational shaft.
9. The device of claim 1 , wherein the support portion and the at least two reaction portions are detachable from the device for real-time measurement of bacteria.
10. The device of claim 1 , further comprising a detector configured to detect a reaction occurring in each of the reaction portions.
11. The device of claim 10 , wherein the detector is a luminometer.
12. A method of measuring bacteria in real time by using the device of claim 1 , the method comprising:
supplying a sample from a sample supply portion to a first reaction portion;
moving a support portion using a rotational shaft, thereby placing a second reaction portion at a position where the second reaction portion is able to receive the sample from the sample supply portion;
detecting ATP present in the first reaction portion; and
repeating the above processes at least once by using at least two reaction portions.