Recombinant AAV1, AAV5, and AAV6 capsid mutants and uses thereof
Provided herein are modified recombinant adeno-associated virus (rAAV) capsid proteins, such as modified rAAV1, rAAV5, and rAAV6 capsid proteins, rAAV particles comprising such capsid proteins, nucleic acid molecules encoding such capsid proteins, as well as compositions, kits and methods of use thereof.
1. A modified adeno-associated virus (AAV) capsid protein, wherein a VP3 region of the modified AAV capsid protein comprises a replacement of tyrosine residues with non-tyrosine residues at positions corresponding to:
Y436, Y693, and Y719 of a wild-type AAV5 capsid protein having the sequence of SEQ ID NO: 2.
2. The modified AAV capsid protein of claim 1 , wherein the modified AAV capsid protein is a modified AAV5 capsid protein and the modified AAV5 capsid protein comprises replacement of tyrosine residues with non-tyrosine residues at each of the positions corresponding to Y436, Y693, and Y719 of a wild-type AAV5 capsid protein having the sequence of SEQ ID NO: 2.
3. The modified capsid protein of claim 1 , wherein the non-tyrosine residues are phenylalanine.
4. A nucleic acid molecule encoding the modified capsid protein of claim 1 .
5. The nucleic acid molecule of claim 4 , wherein the nucleic acid molecule is a plasmid.
6. A recombinant AAV (rAAV) particle comprising the modified capsid protein of claim 1 .
7. A composition comprising a rAAV particle of claim 6 and a pharmaceutically-acceptable carrier.
8. A method, comprising:
contacting a host cell with an rAAV particle comprising the modified AAV capsid protein of claim 1 or a composition comprising the rAAV particle and a pharmaceutically-acceptable carrier.
9. The method of claim 8 , wherein the host cell is a hematopoietic stem cell, dendritic cell, a monocyte, a liver cell, a retinal cell or an airway epithelial cell.
10. A method, comprising:
contacting a host cell with an rAAV particle comprising the modified AAV5 capsid protein of claim 2 or a composition comprising the rAAV particle and a pharmaceutically-acceptable carrier.
11. The method of claim 10 , wherein the host cell is a retinal or airway epithelial cell.
12. A method, comprising:
contacting a host cell with an rAAV particle comprising the modified AAV capsid protein of claim 3 or a composition comprising the rAAV particle and a pharmaceutically-acceptable carrier.
13. The method of claim 12 , wherein the host cell is a hematopoietic stem cell, a dendritic cell, a monocyte, a retinal cell, an airway epithelial cell, a muscle cell, a liver cell, or a microglial cell.
14. A kit comprising the rAAV particle of claim 6 .
15. The modified AAV capsid protein of claim 1 , wherein the AAV capsid is of serotype AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, or AAV13.
16. The modified AAV capsid protein of claim 3 , wherein the modified AAV capsid protein is a modified AAV5 capsid protein and the modified AAV5 capsid protein comprises replacement of tyrosine residues with non-tyrosine residues at each of the positions corresponding to Y436, Y693, and Y719 of a wild-type AAV5 capsid protein having the sequence of SEQ ID NO: 2.
17. The recombinant AAV particle of claim 6 , wherein the AAV capsid is of serotype AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV12, or AAV13.