Method for preparing sugar, bioethanol or microbial metabolite from lignocellulosic biomass
The present invention relates to a method for preparing bioethanol from lignocellulosic biomass. The method of the present invention is capable of: minimizing the impurity content of an enzymatic saccharification raw material, by extracting biomass using hot water, before pretreatment, and removing extractable substances such as inorganic salts; suppressing, to the greatest extent, the production of overdecomposition products of sugar, by pretreating the biomass, from which the hot water extractable substances have been removed, in a condition for maximizing xylan yield; preparing fermentable sugar at a low cost, without washing a pretreated solid obtained from subsequent solid-liquid separation, but by only concentrating a sugar solution obtained after enzymatic saccharification, using a separation film; and preparing bioethanol therefrom in high yield.
1. A method for preparing a sugar solution, the method comprising the steps of:
modifying a polyamide nanofiltration membrane by dipping a polyamide nanofiltration membrane in an aqueous solution containing 0.5 to 3 wt % of sodium hypochlorite and 0.05 to 0.5 wt % of polyethylene glycol methacrylate (step 1); and
filtering an aqueous sugar solution obtained by hydrolyzing cellulosic biomass using the modified polyamide nanofiltration membrane to recover a refined sugar solution from the non-permeate side and remove fermentation inhibitors from the permeate side (step 2).
2. The method according to claim 1 , between the step 1) and the step 2), further comprising:
filtering the aqueous sugar solution obtained by hydrolyzing cellulosic biomass using a microfiltration membrane or ultrafiltration membrane to recover a sugar solution from the permeate side.
3. The method according to claim 1 , after the step 2), further comprising:
filtering the refined sugar solution using a reverse osmosis membrane to recover a refined sugar solution from the non-permeate side and remove fermentation inhibitors from the permeate side.
4. The method according to claim 1 , wherein the fermentation inhibitors are at least one selected from the group consisting of organic acids, furan compounds, and phenol compounds.
5. The method according to claim 1 , wherein the filtration at the step 2) is constant volume filtration.
6. The method according to claim 1 , wherein pH of the aqueous sugar solution at the step 2) is from 4 to 8.
7. The method according to claim 1 , wherein temperature of the aqueous sugar solution at the step 2) is from 15 to 40° C.