Portable device for detecting molecule(s)
An optical assembly 140 for a portable device for detecting molecule(s) within reaction vessels 110 comprises a collimator 403 , a beam splitter arrangement and a plurality of guide arrangements 143 . The collimator 403 collimates an excitation beam from an excitation source 400 . The beam splitter arrangement splits the excitation beam from the collimator into a plurality of split excitation beams. Each beam splitter splits an incoming beam into two beams. In the case where the beam splitter arrangement comprises more than one beam splitter, the beam splitters are arranged in tiers such that a first tier comprises one beam splitter for receiving the excitation beam from the collimator, and each of the other tiers comprises one or more beam splitters. Each beam splitter in at least one of the other tiers receives a split excitation beam from a previous tier. Each guide arrangement 140 guides a respective one of the plurality of split excitation beams along an excitation path A from the beam splitter arrangement into a reaction vessel 110 containing a sample to stimulate an emission of a reaction light from the sample. Each guide arrangement 140 further guides reaction light from the sample along a detection path B towards a detector arrangement 142.
1. A device for detecting molecule(s), the device comprising a sample chamber adapted to receive and contact a reaction vessel, the sample chamber comprising:
a vessel receptacle thermally coupled to a heat exchange device, the vessel receptacle being shaped to receive and substantially encompass the reaction vessel, and having a relatively high thermal conductivity and low thermal mass;
a housing substantially enclosing the vessel receptacle and having an aperture at or substantially adjacent an open end of the vessel receptacle to permit insertion of the reaction vessel in the vessel receptacle;
wherein the vessel receptacle is substantially insulated from the housing;
wherein the heat exchange device comprises a thermoelectric cooling device positioned to be spaced from the sample chamber of a reaction vessel received on the vessel receptacle, wherein the thermoelectric cooling device has a first side that is in thermal communication with the vessel receptacle and a second side that is in thermal communication with a heat sink, wherein the heat sink is configured as a thermal reservoir that is maintained at a substantially constant temperature or within a substantially constant temperature range; and
wherein the device further comprises:
a controller associated with the heat exchange device and configured to control a temperature profile of the sample chamber to perform a reaction;
an excitation assembly for inducing a reaction light in a reaction mixture in the reaction vessel; and
a detector for optically detecting the reaction light.
2. The device for detecting molecule(s) of claim 1 , wherein the heat sink is maintained at a substantially constant temperature or within a substantially constant temperature range above ambient temperature.
3. The device for detecting molecule(s) of claim 2 , wherein the heat sink is maintained within a temperature range of 30-40° C.
4. The device for detecting molecule(s) of claim 1 , wherein the thermoelectric cooling device is adapted to vary the temperature of the vessel receptacle by transferring heat between the heat sink and the vessel receptacle.
5. The device for detecting molecule(s) of claim 1 , wherein the thermoelectric cooling device is a single-stage thermoelectric cooling device.
6. The device for detecting molecule(s) of claim 5 , comprising a plurality of single-stage thermoelectric cooling devices having a first side in thermal communication with the vessel receptacle and a second side in thermal communication with the heat sink.
7. The device for detecting molecule(s) of claim 1 , wherein the vessel receptacle comprises a substantially conical body having a bore for receiving the reaction vessel, and a substantially planar base that is thermally coupled with the heat exchange device, wherein the conical body diverges from the planar base towards the open end.
8. The device for detecting molecule(s) of claim 1 , wherein the vessel receptacle comprises copper, silver, or aluminium, or a combination thereof.
9. The device for detecting molecule(s) of claim 1 , wherein thermal conductivity of the vessel receptacle is higher than 200 Wm −1 K −1 .
10. The device for detecting molecule(s) of claim 1 , wherein the vessel receptacle has a specific heat capacity of up to about 1.0 Jg −1 K −1 at 25° C.
11. The device for detecting molecule(s) of claim 1 , wherein the vessel receptacle is configured to receive a plurality of reaction vessels.
12. The device for detecting molecule(s) of claim 1 , wherein the vessel receptacle is substantially insulated from the housing via an air gap.
13. The device for detecting molecule(s) of claim 1 , wherein an insulative material is provided between the vessel receptacle and the housing.
14. The device for detecting molecule(s) of claim 1 , wherein the controller comprises a microcontroller communicatively coupled to a controller of the heat exchange device and a temperature sensor at or adjacent the vessel receptacle, forming a closed-loop feedback control system.
15. The device for detecting molecule(s) of claim 1 , wherein the excitation assembly and detector are each adapted to be optically coupled with a reaction vessel via the aperture in the housing, by way of respective excitation and detection optical paths.
16. The device for detecting molecule(s) of claim 15 , wherein both or either of the excitation and detection optical paths are folded.
17. The device for detecting molecule(s) of claim 16 , comprising a dichroic mirror that allows the reaction light emitted from within the reaction vessel substantially co-axial with the aperture to pass substantially without reflection or refraction.
18. The device for detecting molecule(s) of claim 1 , wherein the device is suitable or configured for one or more of amplification of nucleic acids including analysis of polymerase chain reactions including quantitative polymerase chain reactions, protein analysis, ligand analysis, or fluorescence analysis of chemical reactions.
19. A portable device for quantitative polymerase chain reactions, the device comprising a sample chamber adapted to receive and contact a reaction vessel, the sample chamber comprising:
a vessel receptacle thermally coupled to a heat exchange device, the vessel receptacle being shaped to receive and substantially encompass a reaction vessel, wherein the heat exchange device comprises a thermoelectric cooling device positioned to be spaced from the sample chamber of a reaction vessel when received on the vessel receptacle, wherein the thermoelectric cooling device has a first side that is in thermal communication with the vessel receptacle and a second side that is in thermal communication with a heat sink, wherein the heat sink is configured as a thermal reservoir that is maintained at a substantially constant temperature or within a substantially constant temperature range; and
wherein the portable device further comprises:
a controller that is configured to vary the temperature of the vessel receptacle by transferring heat between the heat sink and the vessel receptacle to perform a reaction;
an excitation assembly for inducing a reaction light in a reaction mixture in the reaction vessel; and
a detector for optically detecting the reaction light.