Method for identifying, expanding, and removing adult stem cells and cancer stem cells
The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.
1. A method for testing the effect of a compound comprising
(a) contacting a cell population comprising cancer stem cells in vitro with said compound, wherein the cancer stem cells
comprise Lgr6 and/or Lgr5 embedded in their cell membrane;
and
(b) testing the ability of said contacted cancer stem cells to generate a tumor relative to cancer stem cells that are not contacted with said compound,
wherein a difference in the tumor-generating ability of the contacted and non-contacted cancer stem cells indicates an effect of the compound.
2. The method of claim 1 , further comprising testing the effect of said compound on normal tissue stem cells or organ stem cells, comprising contacting normal tissue stem cells or organ stem cells with said compound and determining a change in the number of normal tissue stem cells or organ stem cells, following contact with said compound.
3. The method of claim 1 , wherein the cell population comprises at least 50% Lgr5 and/or Lgr6 positive stem cells, at least 60% Lgr5 and/or Lgr6 positive stem cells, at least 70% Lgr5 and/or Lgr6 positive stem cells, at least 80% Lgr5 and/or Lgr6 positive stem cells, at least 90% Lgr5 and/or Lgr6 positive stem cells, or at least 95% Lgr5 and/or Lgr6 positive stem cells.
4. The method of claim 1 , wherein said compound is an anti-Lgr5 antibody.
5. The method of claim 1 , further comprising measuring the number of cancer stem cells in the cell population before and after contact with said compound.
6. The method of claim 1 , further comprising, prior to step (a), obtaining the cell population comprising cancer stem cells by contacting a cell suspension prepared from a solid or liquid tumor sample with an Lgr6 specific antibody or antibody fragment, and isolating cells that bind to the Lgr6 specific antibody or antibody fragment.