Chitosan-based hemostatic member for cervix and method of manufacturing the same
This invention relates to a hemostatic member using chitosan having superior hemostatic activity, and more particularly to a chitosan-based hemostatic member for the cervix, suitable for use through insertion into an affected part upon cervical biopsy or surgery, configured to include a hemostatic pack having a projection at an upper end thereof through tying with a thread so as to form a spherical shape using chitosan nonwoven fabrics having a hemostatic function, wherein the thread contains an X-ray-sensitive material, thereby realizing effective hemostasis of the affected part upon cervical biopsy or surgery.
1. A chitosan-based hemostatic member, comprising: (1) a hemostatic pack made of chitosan nonwoven fabrics formed in a spherical shape and having a projection at an upper end thereof, said projection the result of the chitosan nonwoven fabrics having been tied with a thread; and (2) peripheral pressing portions,
wherein the peripheral pressing portions comprise a non-chitosan nonwoven fabric or a chitosan nonwoven fabric in a form of a straight line connected to both sides of the projection,
the thread contains an X-ray-sensitive material and extends for connection to the peripheral pressing portions,
the nonwoven fabrics have a hemostatic function, and
the chitosan-based hemostatic member is suitable for use through insertion into an affected part of a cervix upon cervical biopsy or surgery.
2. The chitosan-based hemostatic member of claim 1 , wherein the thread connected to the peripheral pressing portions extends by passing through each of the peripheral pressing portions from inside the hemostatic pack.
3. A method of manufacturing the chitosan-based hemostatic member of claim 1 for a cervix, comprising:
preparing chitosan nonwoven fabrics composed of chitosan fibers; and
shaping the hemostatic pack having the spherical shape by stacking the chitosan nonwoven fabrics and subjecting edges thereof to gathering together and tying with the thread containing an X-ray-sensitive material so as to form a projection.
4. The method of claim 3 , comprising, after the shaping the hemostatic pack:
forming a CMCS coating layer on a surface of the hemostatic pack by immersing the hemostatic pack in a CMCS solution; and then
forming a CMCS sponge by covering an outer surface of the CMOS coating layer with the CMCS solution.
5. The method of claim 4 , wherein the forming the CMCS sponge comprises:
seating the hemostatic pack having the CMCS coating layer formed thereon in a shaping mold;
introducing the CMCS solution into the shaping mold;
lyophilizing the CMCS solution by placing the shaping mold in a lyophilizer to thus achieve an integration with the hemostatic pack; and
thermally treating a lyophilized body by applying heat thereto.
6. The method of claim 5 , wherein preliminary freezing is performed before the lyophilizing, and
the preliminary freezing is carried out in a manner in which the shaping mold is stabilized at 0-−5° C. for 1-2 hr and then immersed for 15 min to 30 min in ethanol cooled to −80° C. or less and thus instantly treated.
7. The method of claim 5 , wherein preliminary freezing is performed before the lyophilizing, and
the preliminary freezing is carried out in a manner in which the shaping mold is placed in the lyophilizer and stabilized in a supercooled state at 0° C.-− 5 ° C. for 2-3 hr and then treated at −30° C.-−40° C. for 3-4 hr.
8. The method of claim 4 , wherein the CMCS solution is prepared by dissolving 3-5% CMCS in distilled water, adding 1-3% glycerin thereto, and performing stirring for 2 hr or more.
9. The method of claim 8 , wherein the CMCS solution obtained through stirring is filtered using a mesh filter so as to increase purity, and is stored at 2-4° C. so as to be defoamed.