Tissue graft
The present invention is directed to a method of producing a tissue graft, comprising at least steps of providing a gel, seeding the gel with cells of at least a first and/or cells of a second type, and culturing of the cells of the first and/or cells of the second type in said gel until the formation of at least one first biostructure in the gel by the cells of the first type and/or the cells of the second type.
1. A dermo-epidermal skin graft comprising a gel, the gel comprising at least one first biostructure formed of cells of at least a first type, and cells of a second type in or on the gel, and a second biostructure comprising cells of at least a third type in or on the gel,
wherein the gel is a compacted hydrogel, and
wherein the cells comprised in the first biostructure are SVF cells or a mixture of endothelial cells and fibroblasts, and
wherein the cells comprised in the second biostructure are keratinocytes,
wherein the first biostructure is a vascular biostructure, and
wherein the second biostructure is an epidermal biostructure.
2. The dermo-epidermal skin graft according to claim 1 , wherein the cells of the first or the second or the third type are of human origin.
3. The dermo-epidermal skin graft according to claim 1 , wherein the tissue graft comprises autologous human endothelial cells and autologous human fibroblasts in the gel.
4. The dermo-epidermal skin graft according to claim 1 , wherein the first biostructure is a vascular biostructure in the gel and the second biostructure is an epidermal biostructure on the gel.
5. The dermo-epidermal skin graft according to claim 1 , comprising lumen-forming lymphatic capillaries.
6. The dermo-epidermal skin graft according to claim 1 , wherein the gel is a fibrin or collagen hydrogel.
7. The dermo-epidermal skin graft according to claim 1 , wherein the graft has a thickness of 0.2-3 mm.
8. The dermo-epidermal skin graft according to claim 1 , wherein the compacted hydrogel is compressed.
9. The dermo-epidermal skin graft according to claim 1 , wherein the second biostructure is formed of cells of at least a second and/or a third type on the gel.
10. The dermo-epidermal skin graft according to claim 2 , wherein the cells of the first or the second or the third type are of autologous human origin.
11. The dermo-epidermal skin graft according to claim 1 , wherein the first or second biostructure are a blood or lymphatic vessel structure.
12. The dermo-epidermal skin graft according to claim 3 , wherein the autologous human endothelial cells and autologous human fibroblasts in the gel are present at a ratio of fibroblasts to endothelial cells of at least 3:7.
13. The dermo-epidermal skin graft according to claim 1 , wherein the vascular biostructure is a vascular plexus of blood and lymphatic capillaries.
14. The dermo-epidermal skin graft according to claim 1 , wherein the graft has a physiological size is 17-60 μm.
15. The dermo-epidermal skin graft according to claim 5 , wherein the lymphatic capillaries have anchoring filament.
16. The dermo-epidermal skin graft according to claim 15 , wherein the anchoring filaments are fibrillin anchoring filaments.
17. The dermo-epidermal skin graft according to claim 6 , wherein the collagen hydrogel is a collagen type I hydrogel.
18. The dermo-epidermal skin graft according to claim 1 , wherein the keratinocytes are human epidermal keratinocytes.
19. The dermo-epidermal skin graft according to claim 7 , wherein the graft has a thickness of 0.7-1 mm.52.
20. The dermo-epidermal skin graft according to claim 5 , wherein the lymphatic capillaries have a continuous lumen of physiological size.