Method for extracting contaminants from agrarian products and apparatus therefor

1. A method for quantitatively extracting contaminants selected from the group consisting of mycotoxins, allergens and genetically modified organisms from agrarian products or foods or animal feeds containing agrarian products, comprising the step of:

providing the agrarian products or foods or animal feeds containing agrarian products that contain or absorbed contaminants selected from the group consisting of mycotoxins, allergens and genetically modified organisms,

providing at least one extraction buffer in a film that is water soluble,

contacting distilled water, the at least one extraction buffer, at least one surfactant and a powdery carrier with the agrarian products or the foods or animal feeds containing agrarian products, in a closable mixing vessel,

closing the closable mixing vessel,

agitating or stirring the mixing vessel so that the film is dissolved and the at least one extraction buffer and the contaminants are brought into solution, and

extracting the contaminants from the agrarian products or foods or animal feeds containing agrarian products;

wherein the distilled water has a temperature 8° C. and 25° C.; and

wherein the at least one extraction buffer selected from the group consisting of borate buffers, carbonate buffers, citrate buffers, phosphate buffers and Tris/HCl buffer.

2. The method according to claim 1 , wherein the extraction buffer is used at a concentration of between 10 mM and 100 mM.

3. The method according to claim 1 , wherein an inert silicate powder is used as said powdery carrier.

4. The method according to claim 3 , wherein the surfactant is selected from polyoxyethyelene lauryl ether, polyoxyethylene acetyl ether, 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol, (1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol, or sodium dodecyl sulfate.

5. The method according to claim 3 , wherein the inert silicate powder is a high purity silicic acid.

6. The method according to claim 1 , wherein the at least one buffer comprises at least two of the following components: sodium or potassium salts of boric acid, carboxylic acid, citric acid, phosphoric acid, phosphorous acid, TRIS (Tris(hydroxymethyl)-aminomethane) and NaCl, said buffer after dissolution having a molarity of 6 to 250 mM, and a pH ranging from 6.5 to 9.5.

7. The method according to claim 1 , wherein the surfactant is non-ionic.

8. The method according to claim 1 , wherein the buffer after dissolution having a molarity of 10 to 100 mM, and a pH ranging between 7.1 and 9.1.

9. The method according to claim 1 , wherein the at least one extraction buffer selected from the group consisting of borate buffers having a pH of 8.5, carbonate buffers having a pH of 9.1, citrate buffers having a pH of 7.1, phosphate buffers having a pH of 7.4 and Tris/HCl buffer having a pH of 7.4.

10. The method according to claim 1 , wherein the agrarian product is maize and the contaminant is mycotoxins, aflatoxins, deoxynivalenol, fumonisins or zearalenones.

11. The method according to claim 1 , wherein the surfactant is selected from polyoxyethyelene lauryl ether, polyoxyethylene acetyl ether, 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol, (1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol, or sodium dodecyl sulfate; and wherein the at least one extraction buffer selected from the group consisting of borate buffers having a pH of 8.5, carbonate buffers having a pH of 9.1, citrate buffers having a pH of 7.1, phosphate buffers having a pH of 7.4 and Tris/HCl buffer having a pH of 7.4.

12. The method according to claim 11 , wherein the method further comprises the step of detecting an amount of the contaminant.