Vaccine compositions
The invention relates to an immunogenic composition comprising influenza virus antigens derived from at least 5 influenza virus strains and one or more compounds of formula (I) or physiologically acceptable salts or esters thereof: wherein: R 1 represents hydrogen or C 1-6 alkyl; R 2 represents C 1-6 alkyl; and R 3 represents C 1-6 alkyl. The invention also relates to methods of preparing the immunogenic composition, to pharmaceutical and vaccine compositions comprising the immunogenic composition and to use of the immunogenic, pharmaceutical and vaccine compositions for preventing influenza virus infection.
1. An immunogenic composition comprising influenza virus antigens derived from at least 5 influenza virus strains and one or more compounds of formula (I) or a physiologically acceptable salt or ester thereof:
wherein:
R 1 represents hydrogen or C 1-6 alkyl;
R 2 represents C 1-6 alkyl; and
R 3 represents C 1-6 alkyl.
2. The immunogenic composition according to claim 1 , comprising influenza virus antigens derived:
from 20 or less influenza virus strains, or
from at least 6 influenza virus strains, or
from 8 to 18 influenza virus strains, or
from 14 to 18 influenza virus strains, or
from 15 influenza virus strains, or
at least 2 influenza virus types, or
from at least 2 subtypes of influenza virus type A.
3. The immunogenic composition according to claim 1 , comprising at least one influenza virus antigen derived from a type A influenza virus strain and at least one influenza virus antigen derived a type B influenza virus strain.
4. The immunogenic composition according to claim 1 , comprising influenza virus antigens derived from at least 2 subtypes of influenza virus type A.
5. The immunogenic composition according to claim 1 , comprising at least one influenza virus antigen derived from a H1N1 subtype of a type A influenza virus strain and at least one influenza virus antigen derived a H3N2 subtype of a type A influenza virus strain.
6. The immunogenic composition according to claim 1 , comprising influenza virus antigens derived from one or more of the following influenza virus strains:
07/102 A/Solomon Islands/3/2006 (H1N1) (IVR-145),
02/336 A/New Caledonia,
08/100 A/Brisbane/59/2007 (IVR-148)(H1N1),
08/140 B/Florida/4/2006,
08/184 B/Malaysia/2506/2004,
09/174 A/California/7/09 (H1N1)(NYMCX-179A),
09/310 A/Wisconsin/15/2009 (H3N2)(NYMCX-183),
12/110 B/Wisconsin/1/2010 (Cell Derived),
12/112 A/Victoria/210/2009 (H3N2)(NYMCX-187),
12/114 A/Victoria/361/2011 (H3N2)(IVR-165),
13/134 B/Massachusetts/02/2012,
13/162 A/Texas/50/2012 (H3N2)(NYMCX-223A),
13/234 B/Brisbane/60/08,
14/252 B/Phuket/3073/2013, and
14/254 A/Switzerland/9715293/2013 (NIB88).
7. The immunogenic composition according to claim 1 , which comprises one or two compounds of formula (I) or physiologically acceptable salts or esters thereof.
8. The immunogenic composition according to claim 1 , which comprises dimethylglycine or a pharmaceutically acceptable salt or ester thereof.
9. The immunogenic composition according to claim 8 , which comprises dimethylglycine and trimethylglycine, or pharmaceutically acceptable salts or esters of one or both thereof.
10. The immunogenic composition according to claim 1 , in which the influenza virus antigens have previously undergone (a) freezing, (b) heat-treating and/or (c) freeze-drying when in an aqueous composition additionally comprising the or each compound.
11. The immunogenic composition according to claim 1 , in which the immunogenic composition further comprises one or more sugars.
12. The immunogenic composition according to claim 1 , in which the immunogenic composition comprises one sugar which is mannitol.
13. The immunogenic composition according to claim 1 , in which the immunogenic composition further comprises an adjuvant.
14. The immunogenic composition according to claim 13 , in which the adjuvant is an aluminium salt adjuvant.
15. An immunogenic composition according to claim 1 , obtainable by a method comprising (a) freezing, (b) heat-treating, and/or (c) freeze-drying (i) a single aqueous composition which comprises the influenza virus antigens and the compound(s), or (ii) two or more aqueous compositions, each of which comprises at least one influenza virus antigen and at least one compound(s), and subsequently combining the resulting treated compositions.
16. A method of inducing an immune response against an influenza virus in a subject, which method comprises administering to the subject an effective amount of an immunogenic composition, the composition comprising influenza virus antigens derived from at least 5 influenza virus strains and one or more compounds of formula (I) or a physiologically acceptable salt or ester thereof:
wherein:
R 1 represents hydrogen or C 1-6 alkyl;
R 2 represents C 1-6 alkyl; and
R 3 represents C 1-6 alkyl.
17. A method of preventing an influenza virus in a subject, which method comprises administering to the subject an effective amount of an immunogenic composition, the composition comprising influenza virus antigens derived from at least 5 influenza virus strains and one or more compounds of formula (I) or a physiologically acceptable salt or ester thereof:
wherein:
R 1 represents hydrogen or C 1-6 alkyl;
R 2 represents C 1-6 alkyl; and
R 3 represents C 1-6 alkyl.