Methods for preparing a dilution series
Methods for preparing a dilution series for use in forming calibration curves, preferably in the field of surface plasmon resonance, are provided. In one example, a dilution series is prepared by using receptacles such as tubes of a micro well plate in which samples of the dilution series are mixed. In another example, a dilution series is prepared by using a convection mix in a receptacle for achieving a concentration gradient in the sample. A biosensor system arranged to perform steps of methods disclosed are provided. A data processing apparatus and a software for performing steps of methods disclosed, and a computer readable medium for storing the software are also provided.
1. A method for preparing a dilution series in a biosensor system, comprising:
a) providing a first sample having a first concentration of a substance and a second sample having a second concentration of said substance,
b) injecting a quantity of the second sample to a measuring device and registering at least one response from a measurement,
c) altering the concentration of the second sample by adding an amount of the first sample to the second sample,
d) injecting a quantity of the second sample generated in step (c) to the measuring device and registering at least one response from a measurement,
e) repeating steps c) and d) until a predetermined number of responses have been registered, said predetermined number of responses being at least three.
2. The method according to claim 1 , wherein the registered responses are used to form a calibration curve.
3. The method according to claim 1 , wherein the predetermined number of responses being at least four.
4. The method Method according to claim 1 , wherein the second concentration is zero.
5. The method according to claim 1 , wherein the substance comprises an organic solvent.
6. The method according to claim 1 , further comprising:
f) Injecting a quantity of the first sample to the measuring device and registering at least one response from a measurement.
7. The method according to claim 2 , wherein the calibration curve is used for surface plasmon resonance.
8. The method of claim 1 , wherein the predetermined number of responses being at least four.
9. The method of claim 5 , wherein the organic solvent is DMSO (dimethyl sulfoxide).